10.1371/journal.pone.0006982.g004
Robbert G. van der Most
Robbert
G. van der Most
Andrew J. Currie
Andrew
J. Currie
Amanda L. Cleaver
Amanda
L. Cleaver
Joanne Salmons
Joanne
Salmons
Anna K. Nowak
Anna
K. Nowak
Sathish Mahendran
Sathish
Mahendran
Irma Larma
Irma
Larma
Amy Prosser
Amy
Prosser
Bruce W. S. Robinson
Bruce
W. S. Robinson
Mark J. Smyth
Mark J.
Smyth
Anthony A. Scalzo
Anthony
A. Scalzo
Mariapia A. Degli-Esposti
Mariapia
A. Degli-Esposti
Richard A. Lake
Richard
A. Lake
Impaired proliferation of CD8 T cells after CY treatment.
Public Library of Science
2013
proliferation
cd8
cells
cy
2013-02-21 04:43:21
Figure
https://plos.figshare.com/articles/figure/_Impaired_proliferation_of_CD8_T_cells_after_CY_treatment_/555057
<p>(A) Draining lymph node cells from tumor-bearing mice treated with saline, CY or gemcitabine (GEM) at day 10 post inoculation were analyzed for Ki-67 expression three days later. Cells shown were gated such that CD8<sup>neg</sup> cells are CD4 T cells. Data shown in lower panel are mean±SEM (<i>n</i> = 3) from one experiment. ** P<0.01 when PBS is compared with CY (unpaired t test). (B) Adoptively transferred transgenic CD8 T cells fail to proliferate in CY-treated mice. CFSE-labeled HA-specific clone 4 cells were transferred into tumor bearing mice two days before PBS or CY injection and were harvested 7 days later and stained for CD8 expression. Cells shown are gated as CD8<sup>+</sup>. Data shown in lower panel are mean±SEM (<i>n</i> = 3) from one experiment. * P<0.05 when PBS is compared with CY (unpaired t test). (C) HA-specific MHC class I pentamer staining in untreated and CY-treated tumor-bearing mice. Data shown are frequencies of pentamer+ CD8 T cells per total CD8 T cells in tumor-draining lymph nodes at day 10 post CY injection. <i>ns</i> = not significant (P = 0.06, unpaired t test). (D) CD43 (clone 1B11) expression on pentamer<sup>+</sup> CD8 T cells in tumor-draining lymph nodes. Cells shown are gated on CD8<sup>+</sup> cells. Representative plots are shown.</p>