10.1371/journal.pone.0006982.g004 Robbert G. van der Most Robbert G. van der Most Andrew J. Currie Andrew J. Currie Amanda L. Cleaver Amanda L. Cleaver Joanne Salmons Joanne Salmons Anna K. Nowak Anna K. Nowak Sathish Mahendran Sathish Mahendran Irma Larma Irma Larma Amy Prosser Amy Prosser Bruce W. S. Robinson Bruce W. S. Robinson Mark J. Smyth Mark J. Smyth Anthony A. Scalzo Anthony A. Scalzo Mariapia A. Degli-Esposti Mariapia A. Degli-Esposti Richard A. Lake Richard A. Lake Impaired proliferation of CD8 T cells after CY treatment. Public Library of Science 2013 proliferation cd8 cells cy 2013-02-21 04:43:21 Figure https://plos.figshare.com/articles/figure/_Impaired_proliferation_of_CD8_T_cells_after_CY_treatment_/555057 <p>(A) Draining lymph node cells from tumor-bearing mice treated with saline, CY or gemcitabine (GEM) at day 10 post inoculation were analyzed for Ki-67 expression three days later. Cells shown were gated such that CD8<sup>neg</sup> cells are CD4 T cells. Data shown in lower panel are mean±SEM (<i>n</i> = 3) from one experiment. ** P<0.01 when PBS is compared with CY (unpaired t test). (B) Adoptively transferred transgenic CD8 T cells fail to proliferate in CY-treated mice. CFSE-labeled HA-specific clone 4 cells were transferred into tumor bearing mice two days before PBS or CY injection and were harvested 7 days later and stained for CD8 expression. Cells shown are gated as CD8<sup>+</sup>. Data shown in lower panel are mean±SEM (<i>n</i> = 3) from one experiment. * P<0.05 when PBS is compared with CY (unpaired t test). (C) HA-specific MHC class I pentamer staining in untreated and CY-treated tumor-bearing mice. Data shown are frequencies of pentamer+ CD8 T cells per total CD8 T cells in tumor-draining lymph nodes at day 10 post CY injection. <i>ns</i> = not significant (P = 0.06, unpaired t test). (D) CD43 (clone 1B11) expression on pentamer<sup>+</sup> CD8 T cells in tumor-draining lymph nodes. Cells shown are gated on CD8<sup>+</sup> cells. Representative plots are shown.</p>