Patterns of pathway activation in gastric cancer cell lines. Huey OoiChia IvanovaTatiana WuJeanie LeeMinghui Beehuat TanIain TaoJiong WardLindsay Hao KooJun GopalakrishnanVeena ZhuYansong Ling ChengLai LeeJulian Young RhaSun Cheol ChungHyun GanesanKumaresan SoJimmy SooKhee Chee LimDennis ChanWeng Hoong WongWai Keong BowtellDavid YeohKhay Guan GrabschHeike BoussioutasAlex TanPatrick 2009 <p>Twenty gene expression signatures representing 11 cancer-related pathways (previously described in <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1000676#pgen-1000676-g002" target="_blank">Figure 2</a>) were queried against a panel of 25 gastric cancer cell lines. The heatmap depicts the activation scores of pathways represented by the signatures (rows) in individual cell lines (columns), with red squares denoting higher activation scores. Pathways and cell lines were ordered using unsupervised hierarchical clustering. Similar to primary tumors, pathways related to proliferation or stem cell form a distinct cluster (brown) from other pathways (grey). Cell lines with high predicted activation of NF-κB, Wnt/β-catenin, or proliferation/stem cell-related pathways are indicated by relevant color bars at the bottom of the heatmap. For the proliferation/stem cell-related signatures, the cell lines were mean-normalized relative to one another against the mean activation score, as all cell lines scored positive for proliferation/stem cell-related pathways.</p>