Relationships
between the Abundance and Expression
of Functional Genes from Vinyl Chloride (VC)-Degrading Bacteria and
Geochemical Parameters at VC-Contaminated Sites
Yi Liang
Xikun Liu
Michael A. Singletary
Kai Wang
Timothy E. Mattes
10.1021/acs.est.7b03521.s001
https://acs.figshare.com/articles/journal_contribution/Relationships_between_the_Abundance_and_Expression_of_Functional_Genes_from_Vinyl_Chloride_VC_-Degrading_Bacteria_and_Geochemical_Parameters_at_VC-Contaminated_Sites/5516962
Bioremediation
of vinyl chloride (VC) contamination in groundwater
could be mediated by three major bacterial guilds: anaerobic VC-dechlorinators,
methanotrophs, and ethene-oxidizing bacteria (etheneotrophs) via metabolic
or cometabolic pathways. We collected 95 groundwater samples across
6 chlorinated ethene-contaminated sites and searched for relationships
among VC biodegradation gene abundance and expression and site geochemical
parameters (e.g., VC concentrations). Functional genes from the three
major VC-degrading bacterial guilds were present in 99% and expressed
in 59% of the samples. Etheneotroph and methanotroph functional gene
abundances ranged from 10<sup>2</sup> to 10<sup>9</sup> genes per
liter of groundwater among the samples with VC reductive dehalogenase
gene (<i>bvcA</i> and <i>vcrA</i>) abundances
reaching 10<sup>8</sup> genes per liter of groundwater. Etheneotroph
functional genes (<i>etnC</i> and <i>etnE</i>)
and VC reductive dehalogenase genes (<i>bvcA</i> and <i>vcrA</i>) were strongly related to VC concentrations (<i>p</i> < 0.001). Methanotroph functional genes (<i>mmoX</i> and <i>pmoA</i>) were not related to VC concentration
(<i>p</i> > 0.05). Samples from sites with bulk VC attenuation
rates >0.08 year<sup>–1</sup> contained higher levels of
etheneotroph
and anaerobic VC-dechlorinator functional genes and transcripts than
those with bulk VC attenuation rates <0.004 year<sup>–1</sup>. We conclude that both etheneotrophs and anaerobic VC-dechlorinators
have the potential to simultaneously contribute to VC biodegradation
at these sites.
2017-10-05 00:00:00
gene abundances
VC concentration
VC biodegradation gene abundance
10 8 genes
Geochemical Parameters
cometabolic pathways
10 2
95 groundwater samples
10 9 genes
VC concentrations
Vinyl Chloride
VC reductive dehalogenase genes
VC reductive dehalogenase gene
VC biodegradation
6 chlorinated ethene-contaminated sites
VC-Contaminated Sites Bioremediation
vinyl chloride
ethene-oxidizing bacteria
Functional genes
site geochemical parameters
Functional Genes