%0 Figure %A Tassi, Elena %A Braga, Marco %A Longhi, Renato %A Gavazzi, Francesca %A Parmiani, Giorgio %A Di Carlo, Valerio %A Pia Protti, Maria %D 2013 %T Effect of titrated doses of IL-12 or IL-27 as single agent on the repertoire of cytokine secreted by CEA177–189/355–367 specific CD4+ T cells. %U https://plos.figshare.com/articles/figure/_Effect_of_titrated_doses_of_IL_12_or_IL_27_as_single_agent_on_the_repertoire_of_cytokine_secreted_by_CEA_177_8211_189_355_8211_367_specific_CD4_T_cells_/551225 %R 10.1371/journal.pone.0007234.g002 %2 https://ndownloader.figshare.com/files/880783 %K titrated %K doses %K il-12 %K il-27 %K repertoire %K cytokine %K secreted %X

(A). CD4+ T cells were cultured with the relevant peptide and irradiated LCL as APC in the presence of increasing concentrations of IL-27 (0-0,1-1-10-100 ng/ml); after 2 days the cytokine release was evaluated by CBA (IL-5 IL-4, and IFN-γ) or ELISA (IL-13). The data for IL-13 are means of duplicate determination±SD. (B). CD4+ T cells were cultured and tested, as described above, in the presence of increasing concentrations of IL-12 (0-5-20 ng/ml). The basal level of cytokines secretion of CD4+ T cells in the presence of LCL only was subtracted from the sample values and was comprised between 0 and 0,018 ng/ml. The data are representative of at least three experiments.

%I PLOS ONE