10.1371/journal.pbio.1000216.g003 Dawen Cai Dawen Cai Dyke P. McEwen Dyke P. McEwen Jeffery R. Martens Jeffery R. Martens Edgar Meyhofer Edgar Meyhofer Kristen J. Verhey Kristen J. Verhey Preferential motility of Kinesin-1 occurs along stable microtubules marked by acetylation and detyrosination of α-tubulin. Public Library of Science 2009 motility kinesin-1 occurs microtubules marked acetylation detyrosination 2009-10-13 00:20:20 Figure https://plos.figshare.com/articles/figure/_Preferential_motility_of_Kinesin_1_occurs_along_stable_microtubules_marked_by_acetylation_and_detyrosination_of_945_tubulin_/551220 <p>Single KHC(1-560)-3xmCit motors in live COS cells were imaged by TIRF microscopy. The cells were fixed and stained for retrospective immunofluorescence. SD Maps of the KHC(1-560)-3xmCit motility events were created from the time series (far left images) and compared to the fixed images of (A) total tubulin (middle panel) and acetylated tubulin (right panel), (B) total tubulin (middle panel) and detyrosinated tubulin (right panel), or (C) total tubulin (middle panel) and polyglutamylated tubulins (right panel). Scale bar, 4 µm. The far right panels indicate schematic representations of the overlap between the Kinesin-1 motility events in the SD Map (green lines) and the modified microtubules (red lines).</p>