TY - DATA T1 - Selective Distance Measurements Using Triple Spin Labeling with Gd3+, Mn2+, and a Nitroxide PY - 2017/10/09 AU - Zuyan Wu AU - Akiva Feintuch AU - Alberto Collauto AU - Luke A. Adams AU - Luigi Aurelio AU - Bim Graham AU - Gottfried Otting AU - Daniella Goldfarb UR - https://acs.figshare.com/articles/journal_contribution/Selective_Distance_Measurements_Using_Triple_Spin_Labeling_with_Gd_sup_3_sup_Mn_sup_2_sup_and_a_Nitroxide/5498101 DO - 10.1021/acs.jpclett.7b01739.s001 L4 - https://ndownloader.figshare.com/files/9512014 KW - biomacromolecule KW - tag KW - Selective Distance Measurements KW - Antennapedia homeodomain orthogonally KW - label KW - nitroxide KW - sample KW - distance constraint KW - assembly KW - dynamics properties KW - tool KW - Mn KW - spectroscopic KW - resonance techniques KW - DNA binding site KW - Nitroxide Distance measurements KW - Triple Spin KW - type KW - Gd KW - pulse electron KW - PELDOR N2 - Distance measurements by pulse electron paramagnetic resonance techniques, such as double electron–electron resonance (DEER, also called PELDOR), have become an established tool to explore structural properties of biomacromolecules and their assemblies. In such measurements a pair of spin labels provides a single distance constraint. Here we show that by employing three different types of spin labels that differ in their spectroscopic and spin dynamics properties it is possible to extract three independent distances from a single sample. We demonstrate this using the Antennapedia homeodomain orthogonally labeled with Gd3+ and Mn2+ tags in complex with its cognate DNA binding site labeled with a nitroxide. ER -