Supplementary Material for: Wnt5a Is Necessary for Normal Kidney Development in Zebrafish and Mice
Huang L.
Xiao A.
Choi S.Y.
Kan Q.
Zhou W.
Chacon-Heszele M.F.
Ryu Y.K.
McKenna S.
Zuo X.
Kuruvilla R.
Lipschutz J.H.
10.6084/m9.figshare.5492854.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Wnt5a_Is_Necessary_for_Normal_Kidney_Development_in_Zebrafish_and_Mice/5492854
<p><b><i>Background:</i></b> Wnt5a is important for the development of
various organs and postnatal cellular function. Little is known,
however, about the role of <i>Wnt5a</i> in kidney development, although <i>WNT5A</i>
mutations were identified in patients with Robinow syndrome, a genetic
disease which includes developmental defects in kidneys. Our goal in
this study was to determine the role of <i>Wnt5a</i> in kidney development. <b><i>Methods:</i></b> Whole-mount in situ hybridization was used to establish the expression pattern of <i>Wnt5a</i> during kidney development. Zebrafish with <i>wnt5a</i> knockdown and <i>Wnt5a</i> global knockout mice were used to identify kidney phenotypes. <b><i>Results:</i></b> In zebrafish, <i>wnt5a</i> knockdown resulted in glomerular cyst formation and dilated renal tubules. In mice, <i>Wnt5a</i>
global knockout resulted in pleiotropic, but severe, kidney phenotypes,
including agenesis, fused kidney, hydronephrosis and duplex
kidney/ureter. <b><i>Conclusions:</i></b> Our data demonstrated the important role of <i>Wnt5a</i>
in kidney development. Disrupted Wnt5a resulted in kidney cysts in
zebrafish and pleiotropic abnormal kidney development in mice.</p>
2017-10-12 13:52:40
Wnt5a
Kidney development
Pronephros
Mesonephros
Metanephros