Supplementary Material for: Wnt5a Is Necessary for Normal Kidney Development in Zebrafish and Mice Huang L. Xiao A. Choi S.Y. Kan Q. Zhou W. Chacon-Heszele M.F. Ryu Y.K. McKenna S. Zuo X. Kuruvilla R. Lipschutz J.H. 10.6084/m9.figshare.5492854.v1 https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Wnt5a_Is_Necessary_for_Normal_Kidney_Development_in_Zebrafish_and_Mice/5492854 <p><b><i>Background:</i></b> Wnt5a is important for the development of various organs and postnatal cellular function. Little is known, however, about the role of <i>Wnt5a</i> in kidney development, although <i>WNT5A</i> mutations were identified in patients with Robinow syndrome, a genetic disease which includes developmental defects in kidneys. Our goal in this study was to determine the role of <i>Wnt5a</i> in kidney development. <b><i>Methods:</i></b> Whole-mount in situ hybridization was used to establish the expression pattern of <i>Wnt5a</i> during kidney development. Zebrafish with <i>wnt5a</i> knockdown and <i>Wnt5a</i> global knockout mice were used to identify kidney phenotypes. <b><i>Results:</i></b> In zebrafish, <i>wnt5a</i> knockdown resulted in glomerular cyst formation and dilated renal tubules. In mice, <i>Wnt5a</i> global knockout resulted in pleiotropic, but severe, kidney phenotypes, including agenesis, fused kidney, hydronephrosis and duplex kidney/ureter. <b><i>Conclusions:</i></b> Our data demonstrated the important role of <i>Wnt5a</i> in kidney development. Disrupted Wnt5a resulted in kidney cysts in zebrafish and pleiotropic abnormal kidney development in mice.</p> 2017-10-12 13:52:40 Wnt5a Kidney development Pronephros Mesonephros Metanephros