%0 Journal Article %A Monneau, Yoan R. %A Luo, Lingjie %A Sankaranarayanan, Nehru Viji %A Nagarajan, Balaji %A R. Vivès, Romain %A Baleux, Françoise %A Desai, Umesh R. %A Arenzana-Seidedos, Fernando %A Lortat-Jacob, Hugues %D 2017 %T Supplementary methods, references and figures from Solution structure of CXCL13 and heparan sulfate binding show that GAG binding site and cellular signalling rely on distinct domains %U https://rs.figshare.com/articles/journal_contribution/Supplementary_methods_references_and_figures_from_Solution_structure_of_CXCL13_and_heparan_sulfate_binding_show_that_GAG_binding_site_and_cellular_signalling_rely_on_distinct_domains/5484520 %R 10.6084/m9.figshare.5484520.v1 %2 https://ndownloader.figshare.com/files/9486145 %K chemokine %K glycosaminoglycan %K CXCl13 %K heparan sulfate binding site %K heparan sulfate sequence %X The supplement includes the experimental procedures for the preparation of CXCL13 and biotinilated HS, the NMR, the molecular dynamics and the binding studies. It comprises 12 supplementary figures and a table supporting the NMR-based structural characterization of CXCL13 monomer and dimer, the kinetic analysis of the binding of wt- and mutant- CXCL13 to HS, the conformational space exploration of CXCL13-HS complex, the mass-spectrometry based analysis of the fluorescently tagged CXCL13, the CXCL13 binding to HS+ and HS- cells, the analysis of the CXCR5 expression of splenocytes and transfected CHO cells and a structural comparison of various Chemokine's 40s loop. %I The Royal Society