10.1371/journal.pone.0007722.t001 Maria Miragaia Maria Miragaia Herminia de Lencastre Herminia de Lencastre Francoise Perdreau-Remington Francoise Perdreau-Remington Henry F. Chambers Henry F. Chambers Julie Higashi Julie Higashi Paul M. Sullam Paul M. Sullam Jessica Lin Jessica Lin Kester I. Wong Kester I. Wong Katherine A. King Katherine A. King Michael Otto Michael Otto George F. Sensabaugh George F. Sensabaugh Binh An Diep Binh An Diep Diversity of ACME and SCC<i>mec</i> among <i>S. epidermidis</i>. Public Library of Science 2013 acme 2013-02-21 03:58:59 Dataset https://plos.figshare.com/articles/dataset/_Diversity_of_ACME_and_SCC_mec_among_S_epidermidis_/547686 1<p>“+” methicillin-resistant <i>S. epidermidis</i>; “−”, methicillin-susceptible <i>S. epidermidis</i>; <sup>2</sup>CC, clonal complex, as previously defined by eBURST analysis <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Miragaia2" target="_blank">[15]</a>, <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Miragaia3" target="_blank">[16]</a>; S, singleton; <sup>3</sup>MLST, multilocus sequence typing <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Thomas1" target="_blank">[14]</a>; 7-loci allelic profile listed in parenthesis (<i>arcC-aroE-gtr-mutS-pyrR-tpiA-yqiL</i>); <sup>4</sup>ACME PCR scan method <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone.0007722-Diep2" target="_blank">[5]</a> defines genetic variants within 3 ACME allotypes: ACME-I contains <i>arc</i> and <i>opp-3</i> gene clusters; ACME-II contains <i>arc</i> but not <i>opp-3</i>; and ACME-III contains <i>opp-3</i> but not <i>arc</i>. ACME-neg is negative for both <i>arc</i> and <i>opp-3</i>. Amplicons from the ACME PCR scan for 5 underlined ACME-I.02 variants were used for DNA sequencing and construction of 25-kb contigs. <sup>5</sup>NH, no hybridization with <i>arcCB</i> probe; <i>ClaI</i>-<i>arcCB</i> banding patterns shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007722#pone-0007722-g003" target="_blank">Figure 3</a>; <sup>6</sup>SCC<i>mec</i> typing to identify class A, B, C, and other non-typeable (NT) <i>mec</i> gene complex, and 5 types of <i>ccr</i> gene complex, ccrAB1, ccrAB2, ccrAB3, ccrAB4, ccrC. SCC<i>mec</i> type I contains B/ccrAB1; type II A/ccrAB2; type III A/ccrAB3; type IV B/ccrAB2; and type V C/ccrC.</p>