TY - DATA T1 - Expression of CD150 isoforms in CLL B cells. PY - 2017/10/05 AU - Inna Gordiienko AU - Larysa Shlapatska AU - Valeriia Kholodniuk AU - Lilia Sklyarenko AU - Daniel F. Gluzman AU - Edward A. Clark AU - Svetlana P. Sidorenko UR - https://plos.figshare.com/articles/figure/Expression_of_CD150_isoforms_in_CLL_B_cells_/5475985 DO - 10.1371/journal.pone.0185940.g004 L4 - https://ndownloader.figshare.com/files/9471223 KW - p 38MAPK phosphorylation KW - GSK KW - CD 150 isoform KW - CLL B cells KW - CD 150 cell surface expression KW - Cell surface expression KW - FOXO KW - JNK KW - CD 180 coligation KW - coligation CD 150 KW - CLL cases KW - CD 150 receptor KW - E-BP KW - CD 150 KW - neoplastic CLL B cells propagation KW - CD 150 isoforms KW - CD 180 receptor pathways KW - leukemia B cells KW - CD 180 crosslinking KW - CD 180 KW - ERK KW - ITSM KW - p 70S S 6RP KW - CLL B cells secrete KW - CD 40 receptors KW - mCD 150 isoform KW - IGHV KW - CD 180 receptors KW - RSK KW - CD 180 target KW - cell surface expression N2 - mRNA expression levels of CD150 isoforms were determined in normal CD19+ and CD5+ peripheral blood B cells and CLL samples that were grouped according to csCD150 expression: csCD150- CLL cases (third column) and csCD150+ CLL cases with more than 6% of CD150+ cells (fourth column). (A) Expression levels of the transmembrane canonical mCD150 isoform. (B) Expression of nCD150 isoform with an alternative cytoplasmic tail. (C) Expression of soluble sCD150 isoform lacking a transmembrane region. * p<0.05 compared to CD19+ B cells, ** p<0.05 compared to CD5+ B cells. The qRT-PCR analysis. Expression of all isoforms was normalised to TBP expression levels. Box plots showed quartiles, median, minimum and maximum value. (D) CLL B cells secrete sCD150 in culture media. Western blot analysis of culture supernatants (tracks 2–4), 5x concentrate of culture supernatants (tracks 5–7) from 3 of 9 tested patients. Track 1 –control culture media. (E) The level of sCD150 in CLL patients’ blood sera, ELISA. ER -