%0 Figure %A Gordiienko, Inna %A Shlapatska, Larysa %A Kholodniuk, Valeriia %A Sklyarenko, Lilia %A Gluzman, Daniel F. %A A. Clark, Edward %A P. Sidorenko, Svetlana %D 2017 %T Expression of CD150 isoforms in CLL B cells. %U https://plos.figshare.com/articles/figure/Expression_of_CD150_isoforms_in_CLL_B_cells_/5475985 %R 10.1371/journal.pone.0185940.g004 %2 https://ndownloader.figshare.com/files/9471223 %K p 38MAPK phosphorylation %K GSK %K CD 150 isoform %K CLL B cells %K CD 150 cell surface expression %K Cell surface expression %K FOXO %K JNK %K CD 180 coligation %K coligation CD 150 %K CLL cases %K CD 150 receptor %K E-BP %K CD 150 %K neoplastic CLL B cells propagation %K CD 150 isoforms %K CD 180 receptor pathways %K leukemia B cells %K CD 180 crosslinking %K CD 180 %K ERK %K ITSM %K p 70S S 6RP %K CLL B cells secrete %K CD 40 receptors %K mCD 150 isoform %K IGHV %K CD 180 receptors %K RSK %K CD 180 target %K cell surface expression %X

mRNA expression levels of CD150 isoforms were determined in normal CD19+ and CD5+ peripheral blood B cells and CLL samples that were grouped according to csCD150 expression: csCD150- CLL cases (third column) and csCD150+ CLL cases with more than 6% of CD150+ cells (fourth column). (A) Expression levels of the transmembrane canonical mCD150 isoform. (B) Expression of nCD150 isoform with an alternative cytoplasmic tail. (C) Expression of soluble sCD150 isoform lacking a transmembrane region. * p<0.05 compared to CD19+ B cells, ** p<0.05 compared to CD5+ B cells. The qRT-PCR analysis. Expression of all isoforms was normalised to TBP expression levels. Box plots showed quartiles, median, minimum and maximum value. (D) CLL B cells secrete sCD150 in culture media. Western blot analysis of culture supernatants (tracks 2–4), 5x concentrate of culture supernatants (tracks 5–7) from 3 of 9 tested patients. Track 1 –control culture media. (E) The level of sCD150 in CLL patients’ blood sera, ELISA.

%I PLOS ONE