10.15131/shef.data.5472550.v1
Nicola Green
Nicola
Green
Robin M Delaine-Smitth
Robin M
Delaine-Smitth
Hannah J Askew
Hannah J
Askew
Robert Byers
Robert
Byers
Gwendolen Reilly
Gwendolen
Reilly
Stephen Matcher
Stephen
Matcher
Data for paper "A new mode of contrast in biological second harmonic generation microscopy"
The University of Sheffield
2017
second harmonic generation
two photon imaging
collagen
myosin
wavelength dependency
Biomaterials
Nonlinear Optics and Spectroscopy
Biophysics
2017-10-06 09:20:23
Dataset
https://orda.shef.ac.uk/articles/dataset/Data_for_paper_A_new_mode_of_contrast_in_biological_second_harmonic_generation_microscopy_/5472550
<p>Enhanced image contrast in biological
second harmonic imaging microscopy (SHIM) has previously been reported via
quantitative assessments of forward- to epi-generated signal intensity ratio
and by polarization analysis. Here we demonstrate a new form of contrast: the
material-specific, wavelength-dependence of epi-generated second harmonic
generation (SHG) excitation efficiency, and discriminate collagen and myosin by
ratiometric epi-generated SHG images at 920 nm and 860 nm. </p>
<p>Collagen shows increased SHG
intensity at 920 nm, while little difference is detected between the two for
myosin; allowing SHIM to characterize different SHG-generating components
within a complex biological sample.</p><p><br></p><p>Scientific Reports 7(1) Article number:13331 (2017)</p>
<p>DOI: 10.1038/s41598-017-13752-y</p>