10.6084/m9.figshare.5458960.v1 Mengmeng Guo Mengmeng Guo Muping Yu Muping Yu Xiang Li Xiang Li Maoguo Li Maoguo Li Immobilised cytochrome <i>c</i> on the carbon dots functionalised MWCNTs and its application to hydrogen peroxide detection Taylor & Francis Group 2017 Cytochrome c carbon dots multi-walled carbon nanotubes hydrogen peroxide biosensors 2017-10-02 10:40:20 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Immobilised_cytochrome_i_c_i_on_the_carbon_dots_functionalised_MWCNTs_and_its_application_to_hydrogen_peroxide_detection/5458960 <p>We have prepared the carbon dots (C-dots) by sucrose carbonisation in the presence of sulphuric acid and characterised by transmission electron microscopy, Fourier transform infrared spectra, fluorescence spectroscopy and UV–vis absorption spectra. The resultant C-dots could be used for the activation of CNTs. Cytochrome <i>c</i> immobilised on the functionalised multi-walled carbon nanotube-modified glass carbon electrode was found to promote electron transfer that was investigated by electrochemical methods. The obtained electrode can also be used as a biocatalyser to catalyse the reduction of H<sub>2</sub>O<sub>2</sub>. Under optimal conditions, the constructed H<sub>2</sub>O<sub>2</sub> sensor showed two linear segments and the detection limits were 1.02 and 2.59 µM (<i>S</i>/<i>N</i> = 3). The modified electrode displayed high stability, good reproducibility and excellent selectivity.</p>