10.6084/m9.figshare.5454877.v2
Carole Seidel
Carole
Seidel
Anaïs Kirsch
Anaïs
Kirsch
Caroline Fontana
Caroline
Fontana
Athanase Visvikis
Athanase
Visvikis
Aurélie Remy
Aurélie
Remy
Laurent Gaté
Laurent
Gaté
Christian Darne
Christian
Darne
Yves Guichard
Yves
Guichard
Epigenetic changes in the early stage of silica-induced cell transformation
Taylor & Francis Group
2017
Promotion assay
silica particles
c-myc expression
epigenetic modifications
2017-10-30 14:18:38
Dataset
https://tandf.figshare.com/articles/dataset/Epigenetic_changes_in_the_early_stage_of_silica-induced_cell_transformation/5454877
<p>The increasing use of nanomaterials in numerous domains has led to growing concern about their potential toxicological properties, and the potential risk to human health posed by silica nanoparticles remains under debate. Recent studies proposed that these particles could alter gene expression through the modulation of epigenetic marks, and the possible relationship between particle exposure and these mechanisms could represent a critical factor in carcinogenicity. In this study, using the Bhas 42 cell model, we compare the effects of exposure to two transforming particles, a pyrogenic amorphous silica nanoparticle NM-203 to those of the crystalline silica particle Min-U-Sil<sup>®</sup> 5. Short-term treatment by Min-U-Sil<sup>®</sup> 5 decreased global DNA methylation and increased the expression of the two <i>de novo</i> DNMTs, DNMT3a and DNMT3b. NM-203 treatment affected neither the expression of these enzymes nor DNA methylation. Moreover, modified global histone H4 acetylation status and HDAC protein levels were observed only in the Min-U-Sil<sup>®</sup> 5-treated cells. Finally, both types of particle treatment induced strong c-Myc expression in the early stage of cell transformation and this correlated with enrichment in RNA polymerase II as well as histone active marks on its promoter. Lastly, almost all parameters that were modulated in the early stage were restored in transformed cells suggesting their involvement mainly in the first steps of cell transformation.</p>