10.1371/journal.pone.0008111.g007 Ana Neves-Costa Ana Neves-Costa W. Ryan Will W. Ryan Will Anna T. Vetter Anna T. Vetter J. Ross Miller J. Ross Miller Patrick Varga-Weisz Patrick Varga-Weisz Fun30 regulates chromatin structure at the <i>HMR</i> boundary element Chromatin analysis using micrococcal nuclease digestion and indirect endlabeling. Public Library of Science 2013 regulates chromatin micrococcal nuclease digestion indirect 2013-02-21 03:41:04 Figure https://plos.figshare.com/articles/figure/_Fun30_regulates_chromatin_structure_at_the_HMR_boundary_element_Chromatin_analysis_using_micrococcal_nuclease_digestion_and_indirect_endlabeling_/544497 <p>Spheroplasts were generated from both a wild-type yeast strain and the isogenic <i>fun30</i> mutant strain and incubated briefly in the presence of increasing concentrations of micrococcal nuclease. The purified genomic DNA was then separated on an agarose gel and analyzed after ethidium bromide staining (left panels) or by Southern blot analysis for the <i>HMR</i> boundary region via indirect end-labelling (right panels). Asterisks indicate major digestion products. The position of the tRNA barrier is indicated by a small box. The arrow indicates the direction of transcription of the tRNA. The coding regions for a1 and <i>GIT1</i> lie upstream and downstream, respectively, of the analysed fragment. The molecular size marker (M) is in multiples of 100 bp.</p>