Trousson, Amalia Makoukji, Joelle X. Petit, Patrice Bernard, Sophie Slomianny, Christian Schumacher, Michael Massaad, Charbel Implication of PGC-1α in sPLA2-IIA promoter activity. <p>A/ Implication of PGC-1α in sPLA2-IIA promoter activity. 158N cells were transiently transfected with either 0.2 µg of sPLA2-IIA(1 kb), 0.1 µg of pRSV-βGal plasmids with PGC-1α expression vector, with mock vector or with a siRNA specifically directed against PGC-1α, as indicated. Eighteen hours after transfection, cells were incubated with 25-OH (10<sup>−5</sup> M) for 24 h, and then luciferase and β-galactosidase activities were analyzed. Results are expressed as percentage of the basal activity and they represent the mean +/− SEM of at least four independent experiments performed in duplicate. *p<0.05 when compared between control cells and cells transfected with PGC-1α expression vector or siRNA against PGC-1α using Bonferroni's test after ANOVA. B/ The efficacy of the siRNA was analyzed by RT-PCR. 158N cells were transiently transfected with a siRNA specifically directed against PGC-1α. 24 hours after transfection, total RNA was prepared. RT-PCR experiments were performed using primers recognizing specifically PGC-1α. PCR products were analyzed on agarose gel (2%) and visualized under UV. 18S RNA was detected by specific primers and used to normalize PGC-1α expression levels.</p> spla2-iia;promoter 2013-02-21
    https://plos.figshare.com/articles/figure/_Implication_of_PGC_1_945_in_sPLA2_IIA_promoter_activity_/544490
10.1371/journal.pone.0008080.g004