Chang, Jing Xia, Yuefeng Wasserloos, Karla Deng, Meihong Blose, Kory J. Vorp, David A. Turnquist, Heth R. R. Billiar, Timothy Pitt, Bruce A. Zhang, Ma-Zhong Zhang, Li-Ming Cyclic stretch activates TLR4-dependent signaling (NF-κB translocation) and pro-inflammatory state (IL-6 release into medium). <p>(A) MLE-12 cells were transfected with 50nM TLR-4 specific siRNA or negative control siRNA using Lipofectamine 2000 for 48 h. TLR-4 expression in control group (con), negative control siRNA group (con siRNA) and TLR-4 specific siRNA group (TLR4 siRNA) were detected by western blot and normalized by β-actin (upper graph). (B) Stretch induced NF-κB nucleus translocation were TLR-4 dependent. Total and nuclear NF-κB were measured by Western blot and its nucleus/total NF-κB ratio was analyzed after 6h cyclic stretch in transfected cells. (C) IL-6 secretion in transfected cell media after 6h cyclic stretch was measured by ELISA and was TLR-4 dependent. Each group collected from three wells in a single experiment and the data were presented as mean ± SD from three separate experiments. **P<0.01, ***P<0.001 when compared between groups denoted by horizontal lines.</p> TLR 4;IL -33. rHMGB 1;MLE;pathway;IL -33 synthesis;IL -33 production;stretch-induced TLR 4-dependent IL -33 biosynthesis;CS;intracellular IL -33 expression;epithelial cells Interleukin 33;HMGB 1 2017-09-12
    https://plos.figshare.com/articles/figure/Cyclic_stretch_activates_TLR4-dependent_signaling_NF-_B_translocation_and_pro-inflammatory_state_IL-6_release_into_medium_/5400115
10.1371/journal.pone.0184770.g005