Chang, Jing Xia, Yuefeng Wasserloos, Karla Deng, Meihong Blose, Kory J. Vorp, David A. Turnquist, Heth R. R. Billiar, Timothy Pitt, Bruce A. Zhang, Ma-Zhong Zhang, Li-Ming Cyclic stretch induced increase in cytosolic and nuclear levels of IL-33. <p>Nuclear and cytoplasm protein fractions of MLE-12 cells with (cyclic S) or without (con) 6h stretch were isolated, and mIL-33 in nucleus and cytoplasm were measured by Western blot (A) or ELISA (B). Lamin B and β-actin (A) served as a loading control for nucleus and cytoplasmic protein, respectively. **P<0.01, ***P<0.001 compared with control (con). Each stretching group collected from three wells for a single experiment, the bar graphs illustrate data representative of three independent experiments.</p> TLR 4;IL -33. rHMGB 1;MLE;pathway;IL -33 synthesis;IL -33 production;stretch-induced TLR 4-dependent IL -33 biosynthesis;CS;intracellular IL -33 expression;epithelial cells Interleukin 33;HMGB 1 2017-09-12
    https://plos.figshare.com/articles/figure/Cyclic_stretch_induced_increase_in_cytosolic_and_nuclear_levels_of_IL-33_/5400106
10.1371/journal.pone.0184770.g004