TY - DATA T1 - Optogenetic excitation of ventral tegmental area (VTA) dopaminergic neurons augments place cells’ spiking. PY - 2017/09/12 AU - Omar Mamad AU - Lars Stumpp AU - Harold M. McNamara AU - Charu Ramakrishnan AU - Karl Deisseroth AU - Richard B. Reilly AU - Marian Tsanov UR - https://plos.figshare.com/articles/figure/Optogenetic_excitation_of_ventral_tegmental_area_VTA_dopaminergic_neurons_augments_place_cells_spiking_/5398972 DO - 10.1371/journal.pbio.2002365.g006 L4 - https://ndownloader.figshare.com/files/9385702 KW - ventral tegmental area KW - remap parallels KW - encode locations KW - hippocampu KW - context reward value KW - support future adaptive behavior KW - experience-dependent value KW - reward context KW - experience-dependent place field assembly distribution KW - show place field KW - memory formation KW - tegmental dopaminergic activity KW - place fields KW - hippocampal neurons KW - Hippocampal place cells KW - place cells KW - reward-driven navigation preference KW - CA 1 region KW - Optogenetic manipulation KW - place field assembly distribution encodes KW - population code N2 - (A) Atlas schematic shows optic fiber and tetrodes in the VTA or hippocampal CA1. Images below show channelrhodopsin 2 with yellow fluorescent protein (ChR2-YFP) expression, tyrosine hydroxylase (TH) staining, and their overlay in VTA of TH::Cre rats injected with E123T/T159C virus. (B) Raster plot from 40 repetitions (above) and firing frequency (below) of optically evoked time-locked excitation of a VTA slow-spiking cell. (C) Raster plot from 40 repetitions (above) and spike count of 120 repetitions (below) of a sample place cell. Time 0 indicates the onset of the stimulation protocol. (D) Firing rate of 22 place cells 100 ms after the onset of the stimulation protocol expressed as a percentage of the prestimulation values, for control (left bar) and photostimulation (middle bar), paired t test: t(21) = −3.910, ***p < 0.001; the right bar shows the firing rate (% prestimulation) for 250 ms after photostimulation onset, paired t test: t(21) = −1.767, p = 0.092. Error bars, mean ± SEM. (E) Raster plot and spike count of a slow-spiking interneuron. (F) Spiking cross correlogram between the place cell and the interneuron shown in (C) and (E), respectively. (G) Firing rate of 21 interneurons 100 ms after photostimulation (% prestimulation), for control (left bar) and photostimulation, paired t test: t(20) = 3.002, **p = 0.007. The right bar shows the firing rate for 250 ms after photostimulation onset, paired t test: t(20) = 6.841, ***p < 0.001. Error bars, mean ± SEM. Files dataset is available at Figshare public repository in Tsanov 2016 data / E123T electrophysiology and E123T immunohistology folders https://figshare.com/s/b86a9a111353ba04bd32. ER -