Localization of Macf1a in stage I oocytes. Matias Escobar-Aguirre Hong Zhang Allison Jamieson-Lucy Mary C. Mullins 10.1371/journal.pgen.1006983.g003 https://plos.figshare.com/articles/figure/Localization_of_Macf1a_in_stage_I_oocytes_/5384458 <p>A-C) Macf1a immunostaining (green) in stage I oocytes shows localization nuclearly and within the Bb (A), and later at the cortex during Bb disassembly (B, C). D) Macf1a staining was negative in a <i>macf1a</i><sup><i>sa12708</i></sup> mutant oocyte. E-G) GFP staining in <i>Gt(macf1a–citrine)</i><sup><i>ct68a</i></sup> line also showed Macf1a-Citrine localization to the Bb and at the cortex during disassembly. DAPI (blue/magenta) stains DNA. A-D) N≥ 3 ovaries, 20 isolated WT oocytes were examined (see <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1006983#sec013" target="_blank">methods</a>), 16 oocytes showed Macf1 nuclear staining; 18 <i>macf1a</i><sup><i>sa12708</i></sup> oocytes were examined. E-H) N = 3 ovaries, 9 of 20 <i>Gt(macf1a–citrine)</i><sup><i>ct68a/+</i></sup> oocytes showed nuclear staining (H). Representative images from 3 experiments. Dotted white lines outline the nucleus. Images are a sum of 3 single optical confocal sections. Arrowheads indicate the Bb. Scale bars: 20 μm.</p> 2017-09-07 17:25:46 Bb granule dissociation Bb mRNP granule dissociation use genome editing macf 1a Macf 1a functions Microtubule-actin crosslinking factor 1 actin binding domain Macf 1a localizes Macf 1a oocyte cortex microtubule-actin crosslinking factor 1 giant multi-domain cytoskeletal linker protein ER Bb disassembly MT CRISPR Balbiani body dissociation PRD CK ABD AF AV