Golden-Gate compatible Magnaporthe oryzae protoplast transformation vectors Helen Pennington Mark Youles Sophien Kamoun 10.6084/m9.figshare.5375428.v1 https://figshare.com/articles/dataset/Golden-Gate_compatible_Magnaporthe_oryzae_protoplast_transformation_vectors/5375428 <div>The Golden Gate cloning system uses standardised parts to facilitate the assembly of multiple transcriptional units, to ensure that future work with these genes can be carried out with ease (Patron et al., 2015 New Phytologist, v. 208, p. 13-19).</div><div><br></div><div>Three fungal transformation vectors have been adapted from the pCB1532 vector series (Sweigard et al., 1997. Fungal Genetics Newsletter 44: 52-53). Vector pCB1532B-RFP Addgene #101854 encodes bialaphos/basta/L-phosphinothricin resistance, pCB1532H-RFP #101855 hygromycin resistance and pCB1532S-RFP #101856 sulfonylurea/chlorimuron ethyl resistance. </div><div><br></div><div>Vectors were domesticated through removal of BsaI cloning sites. An RFP-marker was inserted. The RFP is expressed in E. coli, allowing for red-white selection of transformants. The marker is lost during the Golden Gate reaction, as it is replaced by the inserted transcriptional units. Vectors, sequence information and plasmid maps are available from Addgene https://www.addgene.org/plasmids/articles/28191792/ </div><div><br></div> 2017-09-13 08:37:50 Magnaporthe oryzae Magnaporthe grisea Pyricularia oryzae Fungal transformation Genetic modification Golden Gate Modular Cloning Toolbox Golden Gate DNA assembly method Golden Gate DNA assembly plasmid DNA encoding Biochemistry and Cell Biology not elsewhere classified Biotechnology Cell Biology Genetics Infectious Agents Host-Parasite Interactions Microbiology Molecular Biology Pathology Plant Biology