10.6084/m9.figshare.c.3870805_D16.v1
Bork Berghoff
Bork
Berghoff
Torgny Karlsson
Torgny
Karlsson
Thomas Källman
Thomas
Källman
E. Wagner
E.
Wagner
Manfred Grabherr
Manfred
Grabherr
Additional file 9: of RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study
Springer Nature
2017
RNA-seq
Transcriptomics
Normalization
Gene expression
DNA damage
Stress response
2017-09-05 05:00:00
Figure
https://springernature.figshare.com/articles/figure/Additional_file_9_of_RNA-sequence_data_normalization_through_in_silico_prediction_of_reference_genes_the_bacterial_response_to_DNA_damage_as_case_study/5374552
Contribution of in silico predictions and quadratic correction. Shown are the sample groupings for default parameters, as used in our experiment (a); grouping using a linear fit (b); and no predictions and quadratic fit (c). Out of these, only the combination of predictions and quadratic fit achieve the correct grouping. Also shown are the results when supplying a list of six differently expressed (DE) genes as reference genes (d), in which case five genes are rejected; six randomly selected genes also resolve the grouping correctly (e). (TIFF 1271 kb)