10.6084/m9.figshare.c.3870805_D16.v1 Bork Berghoff Bork Berghoff Torgny Karlsson Torgny Karlsson Thomas Källman Thomas Källman E. Wagner E. Wagner Manfred Grabherr Manfred Grabherr Additional file 9: of RNA-sequence data normalization through in silico prediction of reference genes: the bacterial response to DNA damage as case study Springer Nature 2017 RNA-seq Transcriptomics Normalization Gene expression DNA damage Stress response 2017-09-05 05:00:00 Figure https://springernature.figshare.com/articles/figure/Additional_file_9_of_RNA-sequence_data_normalization_through_in_silico_prediction_of_reference_genes_the_bacterial_response_to_DNA_damage_as_case_study/5374552 Contribution of in silico predictions and quadratic correction. Shown are the sample groupings for default parameters, as used in our experiment (a); grouping using a linear fit (b); and no predictions and quadratic fit (c). Out of these, only the combination of predictions and quadratic fit achieve the correct grouping. Also shown are the results when supplying a list of six differently expressed (DE) genes as reference genes (d), in which case five genes are rejected; six randomly selected genes also resolve the grouping correctly (e). (TIFF 1271 kb)