PBCV-1 genomes detected in host chloroplasts, presumably on a direct track to the host nucleus. Elad Milrot Eyal Shimoni Tali Dadosh Katya Rechav Tamar Unger James L. Van Etten Abraham Minsky 10.1371/journal.ppat.1006562.g006 https://plos.figshare.com/articles/figure/PBCV-1_genomes_detected_in_host_chloroplasts_presumably_on_a_direct_track_to_the_host_nucleus_/5355898 <p>A, B. Cells infected with PBCV-1 for 6 min and thin sections were subjected to In Situ hybridization. A. Low magnification view of a cell showing viral DNA inside chloroplasts. B. High magnification view of inset in panel A. Viral DNA is marked by the inset. Viral DNA is located at membrane stacks on a trajectory to the nucleus. C, D. TEM sections of PBCV-1 infected cells at 2 min PI. Empty capsids are detected near chloroplast stacks. Note the discontinuity of the chloroplast membrane stacks (red arrowhead) at the point of DNA ejection. E, F. Thin section of a 2 min PI chlorella-infected cell. E. Low magnification view of a chlorella cell with a virus near the chloroplast in the process of DNA ejection. F. High magnification view of the inset in panel E. Note the discontinuity of the thylakoid membrane stacks (red arrowheads).Nu: nucleus, Ch: chloroplast. Scale bars: A,E: 500 nm; B,F: 100 nm; C, D: 200 nm.</p> 2017-08-29 17:43:10 host photosynthetic membranes genome internalization genome internalization process infection stages PBCV host cell wall eukaryotic-like infection pathway infection stages dsDNA Paramecium bursaria chlorella virus eukaryote-infecting Paramecium bursaria chlorella virus bacteriophage-like replication cycle