10.6084/m9.figshare.5319580.v2 Yohei Niikura Yohei Niikura Risa Kitagawa Risa Kitagawa Hiroo Ogi Hiroo Ogi Katsumi Kitagawa Katsumi Kitagawa SGT1-HSP90 complex is required for CENP-A deposition at centromeres Taylor & Francis Group 2017 cancer cell cycle CENP-A centromere CUL4 E3 ligase epigenetic mark HSP90 kinetochore mitosis SGT1 SUGT1 ubiquitylation 2017-08-30 17:45:14 Journal contribution https://tandf.figshare.com/articles/journal_contribution/SGT1-HSP90_Complex_Is_Required_for_CENP-A_Deposition_at_Centromeres/5319580 <p>The centromere plays an essential role in accurate chromosome segregation, and defects in its function lead to aneuploidy and thus cancer. The centromere-specific histone H3 variant CENP-A is proposed to be the epigenetic mark of the centromere, as active centromeres require CENP-A–containing nucleosomes to direct the recruitment of multiple kinetochore proteins. CENP-A K124 ubiquitylation, mediated by CUL4A-RBX1-COPS8 E3 ligase activity, is required for CENP-A deposition at the centromere. However, the mechanism that controls the E3 ligase activity of the CUL4A-RBX1-COPS8 complex remains obscure. We have discovered that the SGT1-HSP90 complex is required for recognition of CENP-A by COPS8. Thus, the SGT1-HSP90 complex contributes to the E3 ligase activity of the CUL4A complex that is necessary for CENP-A ubiquitylation and CENP-A deposition at the centromere.</p>