18β-GA-d increased the migration of human skin fibroblasts. Chi-Feng Hung Chien-Yu Hsiao Wen-Hao Hsieh Hsin-Ju Li Yi-Ju Tsai Chun-Nan Lin Hsun-Hsien Chang Nan-Lin Wu 10.1371/journal.pone.0182981.g003 https://plos.figshare.com/articles/figure/18_-GA-d_increased_the_migration_of_human_skin_fibroblasts_/5315809 <p>(A) Human dermal fibroblasts were treated with 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM) and observed at different times (6, 12, and 24 h). <i>In vitro</i> scratch wound healing assay was performed. Cell migration was analyzed using a phase-contrast microscope. Scale bar = 200 μm. (B) Human dermal fibroblasts were treated with mitomycin C (mit) (10 μg/ml) and 18β-GA-d (30 μM), and observed 24 h after treatment. Scale bar = 200 μm. (C) Human dermal fibroblasts on the wells with Electric Cell-substrate Impedance Sensing (ECIS) microelectrodes were wounded by a high electrical voltage. The medium was replaced with a new medium containing 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM), and the cell migration was determined by ECIS. The migration of fibroblasts to the wounded area by electrodes was assayed using real-time measurement of the electrical impedance.</p> 2017-08-16 17:29:52 HaCaT keratinocytes 18β- GA derivative-induced expression migration 18β- GA role aquaporin -3 expression aquaporin -3 proliferation fibroblast compound scratch wound healing assay ERK cause cell death