10.1371/journal.pone.0182981.g003
Chi-Feng Hung
Chi-Feng
Hung
Chien-Yu Hsiao
Chien-Yu
Hsiao
Wen-Hao Hsieh
Wen-Hao
Hsieh
Hsin-Ju Li
Hsin-Ju
Li
Yi-Ju Tsai
Yi-Ju
Tsai
Chun-Nan Lin
Chun-Nan
Lin
Hsun-Hsien Chang
Hsun-Hsien
Chang
Nan-Lin Wu
Nan-Lin
Wu
18β-GA-d increased the migration of human skin fibroblasts.
Public Library of Science
2017
HaCaT keratinocytes
18β- GA derivative-induced expression
migration
18β- GA
role
aquaporin -3 expression
aquaporin -3
proliferation
fibroblast
compound
scratch wound healing assay
ERK
cause cell death
2017-08-16 17:29:52
Figure
https://plos.figshare.com/articles/figure/18_-GA-d_increased_the_migration_of_human_skin_fibroblasts_/5315809
<p>(A) Human dermal fibroblasts were treated with 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM) and observed at different times (6, 12, and 24 h). <i>In vitro</i> scratch wound healing assay was performed. Cell migration was analyzed using a phase-contrast microscope. Scale bar = 200 μm. (B) Human dermal fibroblasts were treated with mitomycin C (mit) (10 μg/ml) and 18β-GA-d (30 μM), and observed 24 h after treatment. Scale bar = 200 μm. (C) Human dermal fibroblasts on the wells with Electric Cell-substrate Impedance Sensing (ECIS) microelectrodes were wounded by a high electrical voltage. The medium was replaced with a new medium containing 0.1% THF or different doses of 18β-GA-d (3, 10, and 30 μM), and the cell migration was determined by ECIS. The migration of fibroblasts to the wounded area by electrodes was assayed using real-time measurement of the electrical impedance.</p>