%0 Figure %A Gehne, Nora %A Lamik, Agathe %A Lehmann, Martin %A Haseloff, Reiner F. %A Andjelkovic, Anuska V. %A Blasig, Ingolf E. %D 2017 %T Fluorescently labeled claudin-5 (Cldn5) cross-over endocytosed via clathrin pathway. %U https://plos.figshare.com/articles/figure/Fluorescently_labeled_claudin-5_Cldn5_cross-over_endocytosed_via_clathrin_pathway_/5312854 %R 10.1371/journal.pone.0182106.g005 %2 https://ndownloader.figshare.com/files/9114271 %K Cldn 5 %K Cross-over endocytosis %K TJ %K extracellular loops Claudins %K cross-over endocytosis %K links junctional membrane turnover %K homophilically cross-over endocytosed %K cross-over endocytosed vesicles %K Amino acid substitutions %K cross-over endocytosed Cldn %K F 147A Q 156E %K cross-over endocytosed Cldns %K paracellularly seal endo %X

Cocultures of monotransfected Madin-Darby canine kidney cells II expressing Cldn5 fluorescently labeled with mTurquoise2 (TRQ) or yellow fluorescent protein (YFP). Treatment with inhibitors of caveolae-formation (filipin III, FIII; 2 h), clathrin-mediated endocytosis (chlorpromazine, CPZ; 1 h) and dynamin (dynasore, Dyn; 2 h). Purple: endogenous Cldn1. Relative numbers of TRQ-Cldn5 vesicles after treatment with inhibitors; meanĀ±SEM, n>50. *p<0.05, **p<0.01, ***p<0.001, compared to the untreated control; one-way ANOVA.

%I PLOS ONE