%0 Figure %A Gehne, Nora %A Lamik, Agathe %A Lehmann, Martin %A Haseloff, Reiner F. %A Andjelkovic, Anuska V. %A Blasig, Ingolf E. %D 2017 %T Mutation (mut) in the extracellular loop (ECL) 2 leads to decreased cis- and trans-interaction of claudins (Cldns). %U https://plos.figshare.com/articles/figure/Mutation_sub_mut_sub_in_the_extracellular_loop_ECL_2_leads_to_decreased_i_cis_i_-_and_i_trans_i_-interaction_of_claudins_Cldns_/5312803 %R 10.1371/journal.pone.0182106.g002 %2 https://ndownloader.figshare.com/files/9114073 %K Cldn 5 %K Cross-over endocytosis %K TJ %K extracellular loops Claudins %K cross-over endocytosis %K links junctional membrane turnover %K homophilically cross-over endocytosed %K cross-over endocytosed vesicles %K Amino acid substitutions %K cross-over endocytosed Cldn %K F 147A Q 156E %K cross-over endocytosed Cldns %K paracellularly seal endo %X

A,B:trans-Interactions assessed by transepithelial electrical resistance (TER) and permeability in filter-grown Madin-Darby canine kidney cells II expressing murine Cldn5wild type (wt), Cldn5F147A, Cldn5Q156E or untransfected (-). A: TER-values, n≥8. B: Permeability coefficient for lucifer yellow across the monolayer, n = 9. C: Homophilic cis-interaction between yellow fluorescent protein (YFP)/mTurquoise2 pairs of murine Cldn5wt or Cldn5mut was measured as fluorescence resonance energy transfer (FRET)-efficiency along the plasma membrane between transiently transfected HEK-293 cells. CRFR: Corticotropin-releasing factor receptor 1 (negative control). Values were normalized to Cldn5wt, n>25. D: Mobility of YFP-Cldn5 in the membrane measured by fluorescence recovery after photobleaching (FRAP). MF: mobile fraction, calculated from fitted curve. n>26. Mean±SEM, *p<0.05, **p<0.01, ***p<0.001, ns: not significant. Kruskal-Wallis test with Dunn’s post hoc test. Differences shown compared to Cldn5wt transfected (*) or untransfected cells (#).

%I PLOS ONE