Supplementary Material for: Antibiotic Susceptibility and Molecular Characterization of Escherichia coli O157 Isolates from Urinary Tract Infections
Xie Z.
Dong Z.
Zhu P.
Zhang L.
Chen X.
Dong C.
10.6084/m9.figshare.5311120.v1
https://karger.figshare.com/articles/dataset/Supplementary_Material_for_Antibiotic_Susceptibility_and_Molecular_Characterization_of_Escherichia_coli_O157_Isolates_from_Urinary_Tract_Infections/5311120
<p><b><i>Background:</i></b><i>Escherichia coli O157</i> is an important
food-borne pathogen that can cause diarrhoea, hemorrhagic colitis, and
hemolytic uraemic syndrome. Enterobacterial repetitive intergenic
consensus sequence PCR (ERIC-PCR) and multilocus sequence typing (MLST)
are good methods for molecular typing and the extensive use of
antibiotics is a contributing factor to the increasing incidence of
antimicrobial-resistant for these strains. <b><i>Objectives:</i></b> The aim of this study was to determine the prevalence, antimicrobial resistance, and genetic diversity of <i>E. coli O157</i> based on the prevalence of urinary tract infection (UTI) in Hubei, China. <b><i>Results:</i></b> We obtained 23 (8.07%) <i>E. coli O157</i>
isolates from 285 UTI patients in Hubei, China. All isolates were
subjected to antibiotic susceptibility analysis, and molecular typing
was performed using ERIC-PCR and MLST. Antimicrobial susceptibility
results indicated that most strains were resistant to penicillin
(95.65%), chloramphenicol (73.91%), and ampicillin (69.57%). All
isolates were discovered to be multiresistant (resistance to more than 3
antibiotics). Genetic variability analysis showed that all of the
isolates were grouped into 4 clusters both by ERIC-PCR and MLST. <b><i>Conclusion:</i></b> Our findings demonstrated the presence of <i>E. coli O157</i> in UTIs, provided insights into the dissemination of antibiotic-resistant strains, and improved our knowledge of <i>E. coli O157</i> risk assessment in UTIs.</p>
2017-08-15 09:18:06
Escherichia coli O157
Urinary tract infections
Antibiotic resistance
Enterobacterial repetitive intergenic consensus sequence PCR
Multilocus sequence typing