TY - DATA T1 - Representative flow cytometry gating strategy. PY - 2017/08/14 AU - Marika Orlov AU - Victoria Dmyterko AU - Mark M. Wurfel AU - Carmen Mikacenic UR - https://plos.figshare.com/articles/figure/Representative_flow_cytometry_gating_strategy_/5307907 DO - 10.1371/journal.pone.0182966.g001 L4 - https://ndownloader.figshare.com/files/9106546 KW - Th 17 cells KW - CCR KW - VAP KW - nosocomial pneumonia KW - alveolar neutrophil numbers KW - Th 17 cell percentages KW - BAL fluid KW - PMA KW - alveolar IL -17A KW - IL -17A levels KW - alveolar Th 17 cells KW - Th 17 cell numbers KW - Conclusions Th 17 cells KW - flow cytometric analysis KW - IFN KW - IL -17A N2 - Alveolar cells were surface stained for CD45, CD4, CCR6 and then intracellularly stained for IL-17A and Interferon-gamma (IFN-ɣ). We gated on single cells, CD45+ cells, live CD4+ T cells, and then for either CCR6+IL17A+ cells or total IFN-ɣ producing cells. SSH: Side Scatter Height, SSA: Side Scatter Area. ER -