10.1371/journal.pgen.1006951.g006
Tobias Bohnenpoll
Tobias
Bohnenpoll
Anna B. Wittern
Anna B.
Wittern
Tamrat M. Mamo
Tamrat M.
Mamo
Anna-Carina Weiss
Anna-Carina
Weiss
Carsten Rudat
Carsten
Rudat
Marc-Jens Kleppa
Marc-Jens
Kleppa
Karin Schuster-Gossler
Karin
Schuster-Gossler
Irina Wojahn
Irina
Wojahn
Timo H.-W. Lüdtke
Timo
H.-W. Lüdtke
Mark-Oliver Trowe
Mark-Oliver
Trowe
Andreas Kispert
Andreas
Kispert
<i>Foxf1</i> is a crucial factor for ureter development.
Public Library of Science
2017
differentiation
ureteric bud epithelium
FOXF 1 function
ureteric mesenchyme
epithelial proliferation
FOXF 1
Forkhead transcription factor gene Foxf 1
FOXF 1-BMP module
HH signal transducer Smoothened
CAKUT
SHH
SMO
SMC
exogenous BMP 4
ureter development
mesenchymal tissue compartments
SHH-FOXF
mesenchymal region
2017-08-10 18:21:43
Figure
https://plos.figshare.com/articles/figure/_i_Foxf1_i_is_a_crucial_factor_for_ureter_development_/5301700
<p>(A-H) Histological and molecular analysis of E14.5 control and <i>Tbx18</i><sup><i>cre/+</i></sup>;<i>Hprt</i><sup><i>Foxf1DN/y</i></sup> (<i>Foxf1DN</i>) ureters after 6 d of culture. (A,B) Hematoxylin and Eosin (HE) stainings and (C-H) analysis of cytodifferentiation by immunofluorescence for ACTA2/CDH1 (C,D), TAGLN (E,F) and ΔNP63/UPK1B (G,H) on proximal ureter sections. (I-Q) Histological, cellular and molecular analysis of control and <i>Tbx18</i><sup><i>cre/+</i></sup>;<i>Hprt</i><sup><i>Foxf1DN/y</i></sup> (<i>Foxf1DN</i>) ureters at E12.5 and E14.5. (I) Hematoxylin and Eosin staining on transverse sections of the proximal ureter at E12.5 and E14.5. (J-N) RNA <i>in situ</i> hybridization analysis for <i>Ptch1</i> (J), <i>Bmp4</i> (K), <i>Myocd</i> (L), <i>Myh11</i> (M) and <i>Tagln</i> (N) expression on transverse sections of the proximal ureter at E12.5 and E14.5. (O-Q) Analysis of cell death by the TUNEL assay (O), and of cellular proliferation by the BrdU incorporation assay (P) on transverse sections of the proximal ureter at E12.5 and E14.5. Black circles in H mark the epithelium (UE) and the inner (IM) and outer (OM) mesenchymal compartments of the ureter for which proliferation was quantified. (Q) Quantification of BrdU-positive cells. E12.5 (n = 3), wildtype versus mutant: IM, 0.329±0.007 vs 0.276±0.011, P = 0.002; OM, 0.216±0.015 vs 0.234±0.019, P = 0.310; UE, 0.412±0.016 vs 0.311±0.026, P = 0.004. E14.5 (n = 3), wildtype versus mutant: IM, 0.199±0.013 vs 0.216±0.003, P = 0.153; OM, 0.209±0.005 vs 0.197±0.013, P = 0.288; UE, 0.225±0.014 vs 0.256±0.020, P = 0.144. Values are displayed as mean ± sd. **, P≤0.01; two-tailed Student’s t-test.</p>