10.1371/journal.pgen.1006951.g006 Tobias Bohnenpoll Tobias Bohnenpoll Anna B. Wittern Anna B. Wittern Tamrat M. Mamo Tamrat M. Mamo Anna-Carina Weiss Anna-Carina Weiss Carsten Rudat Carsten Rudat Marc-Jens Kleppa Marc-Jens Kleppa Karin Schuster-Gossler Karin Schuster-Gossler Irina Wojahn Irina Wojahn Timo H.-W. Lüdtke Timo H.-W. Lüdtke Mark-Oliver Trowe Mark-Oliver Trowe Andreas Kispert Andreas Kispert <i>Foxf1</i> is a crucial factor for ureter development. Public Library of Science 2017 differentiation ureteric bud epithelium FOXF 1 function ureteric mesenchyme epithelial proliferation FOXF 1 Forkhead transcription factor gene Foxf 1 FOXF 1-BMP module HH signal transducer Smoothened CAKUT SHH SMO SMC exogenous BMP 4 ureter development mesenchymal tissue compartments SHH-FOXF mesenchymal region 2017-08-10 18:21:43 Figure https://plos.figshare.com/articles/figure/_i_Foxf1_i_is_a_crucial_factor_for_ureter_development_/5301700 <p>(A-H) Histological and molecular analysis of E14.5 control and <i>Tbx18</i><sup><i>cre/+</i></sup>;<i>Hprt</i><sup><i>Foxf1DN/y</i></sup> (<i>Foxf1DN</i>) ureters after 6 d of culture. (A,B) Hematoxylin and Eosin (HE) stainings and (C-H) analysis of cytodifferentiation by immunofluorescence for ACTA2/CDH1 (C,D), TAGLN (E,F) and ΔNP63/UPK1B (G,H) on proximal ureter sections. (I-Q) Histological, cellular and molecular analysis of control and <i>Tbx18</i><sup><i>cre/+</i></sup>;<i>Hprt</i><sup><i>Foxf1DN/y</i></sup> (<i>Foxf1DN</i>) ureters at E12.5 and E14.5. (I) Hematoxylin and Eosin staining on transverse sections of the proximal ureter at E12.5 and E14.5. (J-N) RNA <i>in situ</i> hybridization analysis for <i>Ptch1</i> (J), <i>Bmp4</i> (K), <i>Myocd</i> (L), <i>Myh11</i> (M) and <i>Tagln</i> (N) expression on transverse sections of the proximal ureter at E12.5 and E14.5. (O-Q) Analysis of cell death by the TUNEL assay (O), and of cellular proliferation by the BrdU incorporation assay (P) on transverse sections of the proximal ureter at E12.5 and E14.5. Black circles in H mark the epithelium (UE) and the inner (IM) and outer (OM) mesenchymal compartments of the ureter for which proliferation was quantified. (Q) Quantification of BrdU-positive cells. E12.5 (n = 3), wildtype versus mutant: IM, 0.329±0.007 vs 0.276±0.011, P = 0.002; OM, 0.216±0.015 vs 0.234±0.019, P = 0.310; UE, 0.412±0.016 vs 0.311±0.026, P = 0.004. E14.5 (n = 3), wildtype versus mutant: IM, 0.199±0.013 vs 0.216±0.003, P = 0.153; OM, 0.209±0.005 vs 0.197±0.013, P = 0.288; UE, 0.225±0.014 vs 0.256±0.020, P = 0.144. Values are displayed as mean ± sd. **, P≤0.01; two-tailed Student’s t-test.</p>