Supplementary Material for: Histamine Iontophoresis as in vivo Model to Study Human Skin Inflammation with Minimal Barrier Impairment: Pilot Study Results of Application of the Model to a Sensitive Skin Panel
Falcone D.
Uzunbajakava N.
Richters R.
van de Kerkhof P.C.M.
van Erp P.E.J.
10.6084/m9.figshare.5245360.v1
https://karger.figshare.com/articles/journal_contribution/Supplementary_Material_for_Histamine_Iontophoresis_as_in_vivo_Model_to_Study_Human_Skin_Inflammation_with_Minimal_Barrier_Impairment_Pilot_Study_Results_of_Application_of_the_Model_to_a_Sensitive_Skin_Panel/5245360
<p><b><i>Background/Aims:</i></b> Histamine iontophoresis is known to
elicit itch and a wheal-and-flare reaction; however, its impact on the
skin barrier and underlying compartments has not been thoroughly
evaluated yet. The primary objective of this study was to characterize
that using immunohistochemistry, biophysical measurements, and image
analysis, and secondly, to explore whether skin reactions to this model
differ in sensitive skin (SS). <b><i>Methods:</i></b> Eighteen healthy subjects, <i>n</i> = 9 with SS and <i>n</i>
= 9 with non-sensitive skin (NSS), were included based on a
perception-based questionnaire. Histamine iontophoresis was performed on
the buttock, and skin reactions were evaluated up to 72 h after
stimulation. <b><i>Results:</i></b> The wheal-and-flare peaked at 30
min; after 8 h, no clinical signs were visible. No signs of disruption
of the stratum corneum, as well as no increase in the number of
Ki67-positive cells emerged, whereas fewer tryptase-positive mast cells
and increased epidermal thickness were observed at 1 and 72 h,
respectively. SS subjects showed higher perception of itch compared to
NSS subjects. <b><i>Conclusion:</i></b> Histamine iontophoresis is a
well-standardized in vivo model to quantitatively study the early stages
of cutaneous inflammation with minimal impact on the skin barrier. In
line with previous studies, it highlighted increased sensory perceptions
in SS.</p>
2017-07-26 12:52:22
Histamine
Iontophoresis
Biophysical measurements
Immunohistochemistry
Histology
Image analysis
Skin barrier
In vivo model of cutaneous inflammation
Sensitive skin