Erratum: Overexpression of BDNF and Full-Length TrkB Receptor Ameliorate Striatal Neural Survival in Huntington's Disease Silva A. Naia L. Dominguez A. Ribeiro M. Rodrigues J. Vieira O.V. Lessmann V. Rego A.C. 10.6084/m9.figshare.5241775.v1 https://karger.figshare.com/articles/dataset/Erratum_Overexpression_of_BDNF_and_Full-Length_TrkB_Receptor_Ameliorate_Striatal_Neural_Survival_in_Huntington_s_Disease/5241775 <b><i>Background:</i></b> Several cellular mechanisms have been proposed to explain the pathogenesis of Huntington's disease (HD), including the lack of striatal brain-derived neurotrophic factor (BDNF). Thus, by preferentially binding to tropomyosin receptor kinase B (TrkB) receptor, BDNF is an important neurotrophin implicated in striatal neuronal survival. <b><i>Objective:</i></b> To study the influence of BDNF and TrkB receptors in intracellular signaling pathways and caspase-3 activation in HD striatal cells. <b><i>Methods:</i></b> HD mutant knockin and wild-type striatal cells were transduced with preproBDNF or full-length TrkB receptors to analyze BDNF processing, AKT and extracellular signal-regulated kinase (ERK) activation and the activity of caspase-3 in the absence or presence of staurosporine (STS). <b><i>Results:</i></b> HD mutant cells transduced with preproBDNF-mCherry (mCh) expressed similar levels of pro- and mature BDNF compared to WT cells, but HD cells released lower levels of pro- and mature BDNF. Despite this, BDNF-mCh overexpression rescued decreased AKT phosphorylation and reduced the caspase-3 activation observed in HD cells. Activated ERK was also enhanced in HD BDNF-mCh/TrkB-eGFP receptor co-cultures. Of relevance, overexpression of TrkB-eGFP in HD cells decreased caspase-3 activation, and stimulation of TrkB-eGFP-transduced mutant cells with recombinant human BDNF reduced both basal and STS-induced caspase-3 activation. <b><i>Conclusion:</i></b> The results highlight the importance of BDNF-induced TrkB receptor signaling in rescuing HD-mediated apoptotic features in striatal cells. 2017-07-25 13:55:43 Huntingtonߣs disease Mutant huntingtin BDNF TrkB receptors Caspase-3 Apoptosis