ICP0 expressed from the D8 mutant localizes exclusively in the cytoplasm. PaladinoPatrick E. CollinsSusan L. MossmanKaren 2010 <p>HEL cells were infected with WT HSV-1 (F strain), R7914 or D8 for 8 hours. ICP0 localization was monitored by immunofluorescence microscopy. Cell nuclei were hoechst stained (DAPI).</p>