Amparo, Tatiane Roquete Rodrigues, Ivanildes Vasconcelos Seibert, Janaína Brandão Souza, Rafaella Hilda Zaniti Ribeiro de Oliveira, Amanda Cabral, Vivette Appolinário Rodrigues Melo de Abreu Vieira, Paula Brandão, Geraldo Célio Okuma, Adriana Akemi Filho, Sidney Augusto Vieira Teixeira, Luiz Fernando Medeiros de Souza, Gustavo Henrique Bianco Antibacterial activity of extract and fractions from branches of <i>Protium spruceanum</i> and cytotoxicity on fibroblasts <p>The crude ethanol extract (CEE) and fractions from branches of <i>Protium spruceanum</i> were subjected to antibacterial and cytotoxicity assays. Compounds of the most active fraction were identified by GC-MS and LC-MS. CEE was active against 19 bacteria and the ethyl acetate fraction (EAF) showed the lowest minimum bactericidal concentration (MBC 0.3–80.0 mg/mL). Through time-kill assay was observed that EAF induced rapid bactericidal effect against <i>Staphylococcus saprophyticus</i>. The cytotoxicity tests against L929 fibroblasts showed great potential of EAF on the treatment of infections caused by five bacteria (MBC < IC<sub>50</sub>). The results provide <i>in vitro</i> scientific support to the possible application of branches of <i>P. spruceanum</i> as antimicrobial agent that may contribute for treatment of infections.</p> Protium spruceanum;antbacterial activity;cytotoxicity 2017-07-20
    https://tandf.figshare.com/articles/journal_contribution/Antibacterial_activity_of_extract_and_fractions_from_branches_of_i_Protium_spruceanum_i_and_cytotoxicity_on_fibroblasts/5226193
10.6084/m9.figshare.5226193.v1