10.1021/acs.jafc.7b02039.s001 Delin Mo Delin Mo Kaifan Yu Kaifan Yu Hu Chen Hu Chen Luxi Chen Luxi Chen Xiaohong Liu Xiaohong Liu Zuyong He Zuyong He Peiqing Cong Peiqing Cong Yaosheng Chen Yaosheng Chen Transcriptome Landscape of Porcine Intramuscular Adipocytes during Differentiation American Chemical Society 2017 mRNA profiles intramuscular adipocytes adipogenic marker PPAR γ POSTN 4 h candidate genes Primary intramuscular preadipocytes intramuscular preadipocyte differentiation AKR 1CL intramuscular adipose tissue transcription factors IMAT formation 2 days transcriptional cascade 4 clusters porcine intramuscular preadipocyte differentiation meat quality Transcriptome Landscape gene expression pattern adipocyte differentiation process transcriptome landscape FGFR 4 1 day 6 days food animals Porcine Intramuscular Adipocytes 8 h 2017-07-04 00:00:00 Journal contribution https://acs.figshare.com/articles/journal_contribution/Transcriptome_Landscape_of_Porcine_Intramuscular_Adipocytes_during_Differentiation/5221489 The adipocyte differentiation process, controlled by a tightly regulated transcriptional cascade, contributes partly to determine intramuscular adipose tissue (IMAT) mass, which is associated with meat quality in food animals, as well as obesity and related metabolic complications in human. Thus, this study aimed to characterize genes critical for intramuscular preadipocyte differentiation. Primary intramuscular preadipocytes were isolated from pigs, and mRNA profiles were performed at several key points (0 h, 4 h, 8 h, 1 day, 2 days, and 6 days) during adipogenesis using microarrays. By gene functional analysis, we identified numerous differentially expressed genes among distinct stages of intramuscular preadipocyte differentiation, which included numbers of transcription factors in the early stages. We obtained 4 clusters of differential gene expression pattern, including crucial candidate genes associated with adipogenesis of intramuscular adipocytes. Further, we demonstrated that <i>POSTN</i> and <i>FGFR4</i> suppressed, whereas <i>AKR1CL1</i> promoted, the expression of adipogenic marker <i>PPARγ</i> and <i>C/EBPα</i>. Taken together, our data delineated the transcriptome landscape during porcine intramuscular preadipocyte differentiation, which provided a valuable resource for finding the genes responsible for IMAT formation.