10.1021/acs.jafc.7b02039.s001
Delin Mo
Delin
Mo
Kaifan Yu
Kaifan
Yu
Hu Chen
Hu
Chen
Luxi Chen
Luxi
Chen
Xiaohong Liu
Xiaohong
Liu
Zuyong He
Zuyong
He
Peiqing Cong
Peiqing
Cong
Yaosheng Chen
Yaosheng
Chen
Transcriptome Landscape of Porcine Intramuscular Adipocytes
during Differentiation
American Chemical Society
2017
mRNA profiles
intramuscular adipocytes
adipogenic marker PPAR γ
POSTN
4 h
candidate genes
Primary intramuscular preadipocytes
intramuscular preadipocyte differentiation
AKR 1CL
intramuscular adipose tissue
transcription factors
IMAT formation
2 days
transcriptional cascade
4 clusters
porcine intramuscular preadipocyte differentiation
meat quality
Transcriptome Landscape
gene expression pattern
adipocyte differentiation process
transcriptome landscape
FGFR 4
1 day
6 days
food animals
Porcine Intramuscular Adipocytes
8 h
2017-07-04 00:00:00
Journal contribution
https://acs.figshare.com/articles/journal_contribution/Transcriptome_Landscape_of_Porcine_Intramuscular_Adipocytes_during_Differentiation/5221489
The adipocyte differentiation process,
controlled by a tightly
regulated transcriptional cascade, contributes partly to determine
intramuscular adipose tissue (IMAT) mass, which is associated with
meat quality in food animals, as well as obesity and related metabolic
complications in human. Thus, this study aimed to characterize genes
critical for intramuscular preadipocyte differentiation. Primary intramuscular
preadipocytes were isolated from pigs, and mRNA profiles were performed
at several key points (0 h, 4 h, 8 h, 1 day, 2 days, and 6 days) during
adipogenesis using microarrays. By gene functional analysis, we identified
numerous differentially expressed genes among distinct stages of intramuscular
preadipocyte differentiation, which included numbers of transcription factors
in the early stages. We obtained 4 clusters of differential
gene expression pattern, including crucial candidate genes associated
with adipogenesis of intramuscular adipocytes. Further, we demonstrated that <i>POSTN</i> and <i>FGFR4</i> suppressed, whereas <i>AKR1CL1</i> promoted,
the expression of adipogenic marker <i>PPARγ</i> and <i>C/EBPα</i>. Taken together, our data delineated the transcriptome
landscape during porcine intramuscular preadipocyte differentiation,
which provided a valuable resource for finding the genes responsible
for IMAT formation.