10.6084/m9.figshare.5212552.v1 Si Yu Si Yu Lijiao Wang Lijiao Wang Zhixing Cao Zhixing Cao Daoyin Gong Daoyin Gong Qianyi Liang Qianyi Liang Hanting Chen Hanting Chen Huizhu Fu Huizhu Fu Wenwen Wang Wenwen Wang Xue Tang Xue Tang Zihao Xie Zihao Xie Yang He Yang He Cheng Peng Cheng Peng Yuzhi Li Yuzhi Li Anticancer effect of Polyphyllin Ι in colorectal cancer cells through ROS-dependent autophagy and G2/M arrest mechanisms Taylor & Francis Group 2017 Polyphyllin Ι autophagy ROS G2/M arrest 2017-07-17 06:17:55 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Anticancer_effect_of_Polyphyllin_in_colorectal_cancer_cells_through_ROS-dependent_autophagy_and_G2_M_arrest_mechanisms/5212552 <p>Polyphyllin Ι is a steroidal saponin isolated from the rhizoma of <i>Paris polyphylla</i>. In the present study, we aimed to investigate the anticancer effects of polyphyllin Ι in colorectal cancer and to elucidate the potential underlying molecular mechanisms. Using, CCK8 assay, flow cytometry, laser confocal microscope analysis and western blot, the anticancer effects of the polyphyllin Ι were analysed in colorectal cells. Our results indicate that polyphyllin Ι significantly decreased cell viability of HCT 116 cells and induced autophagy. Furthermore, we found that polyphyllin Ι induced autophagy in an ROS-dependent cell death and not related with PI3 K/AKT/mTOR pathway. We also provide evidence that excessive ROS triggered by polyphyllin Ι could induce G2/M phase arrest via regulating cycle proteins expression of cell cycle regulators, such as p21 and cyclinB1. In conclusion, polyphyllin Ι exhibit anticancer effect through ROS-dependent autophagy and induces G2/M arrest in colorectal cancer.</p>