10.6084/m9.figshare.5212552.v1
Si Yu
Si
Yu
Lijiao Wang
Lijiao
Wang
Zhixing Cao
Zhixing
Cao
Daoyin Gong
Daoyin
Gong
Qianyi Liang
Qianyi
Liang
Hanting Chen
Hanting
Chen
Huizhu Fu
Huizhu
Fu
Wenwen Wang
Wenwen
Wang
Xue Tang
Xue
Tang
Zihao Xie
Zihao
Xie
Yang He
Yang
He
Cheng Peng
Cheng
Peng
Yuzhi Li
Yuzhi
Li
Anticancer effect of Polyphyllin Ι in colorectal cancer cells through ROS-dependent autophagy and G2/M arrest mechanisms
Taylor & Francis Group
2017
Polyphyllin Ι
autophagy
ROS
G2/M arrest
2017-07-17 06:17:55
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Anticancer_effect_of_Polyphyllin_in_colorectal_cancer_cells_through_ROS-dependent_autophagy_and_G2_M_arrest_mechanisms/5212552
<p>Polyphyllin Ι is a steroidal saponin isolated from the rhizoma of <i>Paris polyphylla</i>. In the present study, we aimed to investigate the anticancer effects of polyphyllin Ι in colorectal cancer and to elucidate the potential underlying molecular mechanisms. Using, CCK8 assay, flow cytometry, laser confocal microscope analysis and western blot, the anticancer effects of the polyphyllin Ι were analysed in colorectal cells. Our results indicate that polyphyllin Ι significantly decreased cell viability of HCT 116 cells and induced autophagy. Furthermore, we found that polyphyllin Ι induced autophagy in an ROS-dependent cell death and not related with PI3 K/AKT/mTOR pathway. We also provide evidence that excessive ROS triggered by polyphyllin Ι could induce G2/M phase arrest via regulating cycle proteins expression of cell cycle regulators, such as p21 and cyclinB1. In conclusion, polyphyllin Ι exhibit anticancer effect through ROS-dependent autophagy and induces G2/M arrest in colorectal cancer.</p>