10.6084/m9.figshare.5212060.v1 Jaipal S. Choudhary Jaipal S. Choudhary Naiyar Naaz Naiyar Naaz Moanaro Lemtur Moanaro Lemtur Bikash Das Bikash Das Arun Kumar Singh Arun Kumar Singh Bhagwati P. Bhatt Bhagwati P. Bhatt Chandra S. Prabhakar Chandra S. Prabhakar Genetic analysis of <i>Bactrocera zonata</i> (Diptera: Tephritidae) populations from India based on <i>cox1</i> and <i>nad1</i> gene sequences Taylor & Francis Group 2017 Bactrocera cox1 gene nad1 gene genetic diversity DNA barcode 2017-07-15 15:56:03 Journal contribution https://tandf.figshare.com/articles/journal_contribution/Genetic_analysis_of_i_Bactrocera_zonata_i_Diptera_Tephritidae_populations_from_India_based_on_i_cox1_i_and_i_nad1_i_gene_sequences/5212060 <p>The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus <i>Bactrocera</i>. In the present study, the genetic structure, diversity and demographic history of <i>B. zonata</i> in India were inferred from mitochondrial <i>cytochrome oxidase 1</i> (<i>cox1</i>) and <i>NADH dehydrogenase 1</i> (<i>nad1</i>) sequences. The efficiency of DNA barcodes for identification of <i>B. zonata</i> was also tested. Genetic diversity indices [number of haplotypes (<i>H</i>), haplotype diversity (<i>Hd</i>), nucleotide diversity (<i>π</i>) and average number of nucleotide differences (<i>k</i>)] of <i>B. zonata</i> populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-significant negative correlation between pairwise <i>F</i>st and geographic distance suggests a high level of gene flow among studied populations of <i>B. zonata</i>. The possibility of sudden expansion of <i>B. zonata</i> revealed through mismatch distribution analysis as well as negative Tajima’s <i>D</i> and Fu’s <i>F</i>s values further supported by star-like network of haplotypes. DNA barcoding analysis suggests that <i>B. zonata</i> specimens can be clearly differentiated from other species with 100% accuracy of identification. Therefore, <i>cytochrome oxidase 1</i> (<i>cox1</i>) barcode sequences generated in the present study could be a valuable source for the rapid identification and global population genetic study of <i>B. zonata.</i></p>