10.6084/m9.figshare.5212060.v1
Jaipal S. Choudhary
Jaipal S.
Choudhary
Naiyar Naaz
Naiyar
Naaz
Moanaro Lemtur
Moanaro
Lemtur
Bikash Das
Bikash
Das
Arun Kumar Singh
Arun Kumar
Singh
Bhagwati P. Bhatt
Bhagwati P.
Bhatt
Chandra S. Prabhakar
Chandra S.
Prabhakar
Genetic analysis of <i>Bactrocera zonata</i> (Diptera: Tephritidae) populations from India based on <i>cox1</i> and <i>nad1</i> gene sequences
Taylor & Francis Group
2017
Bactrocera
cox1 gene
nad1 gene
genetic diversity
DNA barcode
2017-07-15 15:56:03
Journal contribution
https://tandf.figshare.com/articles/journal_contribution/Genetic_analysis_of_i_Bactrocera_zonata_i_Diptera_Tephritidae_populations_from_India_based_on_i_cox1_i_and_i_nad1_i_gene_sequences/5212060
<p>The peach fruit fly, <i>Bactrocera zonata</i>, is among the most serious and polyphagous insect pest of fruit crops in many parts of the world under genus <i>Bactrocera</i>. In the present study, the genetic structure, diversity and demographic history of <i>B. zonata</i> in India were inferred from mitochondrial <i>cytochrome oxidase 1</i> (<i>cox1</i>) and <i>NADH dehydrogenase 1</i> (<i>nad1</i>) sequences. The efficiency of DNA barcodes for identification of <i>B. zonata</i> was also tested. Genetic diversity indices [number of haplotypes (<i>H</i>), haplotype diversity (<i>Hd</i>), nucleotide diversity (<i>π</i>) and average number of nucleotide differences (<i>k</i>)] of <i>B. zonata</i> populations across India maintain high level of genetic diversity without isolation by distance among the geographic regions. Non-significant negative correlation between pairwise <i>F</i>st and geographic distance suggests a high level of gene flow among studied populations of <i>B. zonata</i>. The possibility of sudden expansion of <i>B. zonata</i> revealed through mismatch distribution analysis as well as negative Tajima’s <i>D</i> and Fu’s <i>F</i>s values further supported by star-like network of haplotypes. DNA barcoding analysis suggests that <i>B. zonata</i> specimens can be clearly differentiated from other species with 100% accuracy of identification. Therefore, <i>cytochrome oxidase 1</i> (<i>cox1</i>) barcode sequences generated in the present study could be a valuable source for the rapid identification and global population genetic study of <i>B. zonata.</i></p>