%0 Figure %A Couderc, Elodie %A Morel, Franck %A Levillain, Pierre %A Buffière-Morgado, Amandine %A Camus, Magalie %A Paquier, Camille %A Bodet, Charles %A Jégou, Jean-François %A Pohin, Mathilde %A Favot, Laure %A Garcia, Martine %A Huguier, Vincent %A Mcheik, Jiad %A Lacombe, Corinne %A Yssel, Hans %A Guillet, Gérard %A Bernard, François-Xavier %A Lecron, Jean-Claude %D 2017 %T Expression of proinflammatory mediators by A-SAA-stimulated NHEK and in synergy with IL-17A. %U https://plos.figshare.com/articles/figure/Expression_of_proinflammatory_mediators_by_A-SAA-stimulated_NHEK_and_in_synergy_with_IL-17A_/5211271 %R 10.1371/journal.pone.0181486.g002 %2 https://ndownloader.figshare.com/files/8898943 %K A-SAA expression %K CCR %K CCL %K Interleukin -17A production %K RT %K Th 17 lymphocyte recruitment %K Human Epidermal Keratinocytes %K STAT %K Th 17 lymphocytes %K OSM %K NF %K ELISA %K psoriasis pathogenesis Background Acute-serum Amyloid %K AD %K TNF %K IL %K NHEK %X

(A) NHEK were incubated with A-SAA (10 μg/ml) for 24 hours and the expression of TNF-α, S100A7, S100A8, hBD2, CCL20 and A-SAA was determined by RT-qPCR. (B) IL-17A (10 ng/ml) had a synergistic effect with rA-SAA. After A-SAA and IL-17A costimulation, mRNA expression of S100A7, hBD2 and A-SAA were further increased compared to A-SAA or IL-17A alone. Three independent experiments with duplicates were performed. Values are expressed as mean ± SEM fold change above unstimulated NHEK. Statistical comparisons were performed using t test (*p<0.05; **p<0.01; ***p<0.001).

%I PLOS ONE