10.1371/journal.pone.0181486.g002
Elodie Couderc
Elodie
Couderc
Franck Morel
Franck
Morel
Pierre Levillain
Pierre
Levillain
Amandine Buffière-Morgado
Amandine
Buffière-Morgado
Magalie Camus
Magalie
Camus
Camille Paquier
Camille
Paquier
Charles Bodet
Charles
Bodet
Jean-François Jégou
Jean-François
Jégou
Mathilde Pohin
Mathilde
Pohin
Laure Favot
Laure
Favot
Martine Garcia
Martine
Garcia
Vincent Huguier
Vincent
Huguier
Jiad Mcheik
Jiad
Mcheik
Corinne Lacombe
Corinne
Lacombe
Hans Yssel
Hans
Yssel
Gérard Guillet
Gérard
Guillet
François-Xavier Bernard
François-Xavier
Bernard
Jean-Claude Lecron
Jean-Claude
Lecron
Expression of proinflammatory mediators by A-SAA-stimulated NHEK and in synergy with IL-17A.
Public Library of Science
2017
A-SAA expression
CCR
CCL
Interleukin -17A production
RT
Th 17 lymphocyte recruitment
Human Epidermal Keratinocytes
STAT
Th 17 lymphocytes
OSM
NF
ELISA
psoriasis pathogenesis Background Acute-serum Amyloid
AD
TNF
IL
NHEK
2017-07-14 17:52:42
Figure
https://plos.figshare.com/articles/figure/Expression_of_proinflammatory_mediators_by_A-SAA-stimulated_NHEK_and_in_synergy_with_IL-17A_/5211271
<p>(A) NHEK were incubated with A-SAA (10 μg/ml) for 24 hours and the expression of TNF-α, S100A7, S100A8, hBD2, CCL20 and A-SAA was determined by RT-qPCR. (B) IL-17A (10 ng/ml) had a synergistic effect with rA-SAA. After A-SAA and IL-17A costimulation, mRNA expression of S100A7, hBD2 and A-SAA were further increased compared to A-SAA or IL-17A alone. Three independent experiments with duplicates were performed. Values are expressed as mean ± SEM fold change above unstimulated NHEK. Statistical comparisons were performed using t test (*p<0.05; **p<0.01; ***p<0.001).</p>