%0 Figure %A Ries, Moritz %A R. Reynolds, Matthew %A Bashkueva, Ksenia %A Crosno, Kristin %A Capuano, Saverio %A Prall, Trent M. %A Wiseman, Roger %A H. O’Connor, David %A G. Rakasz, Eva %A Uno, Hajime %A D. Lifson, Jeffrey %A T. Evans, David %D 2017 %T NK cell degranulation and cytokine release in response to SIV infection. %U https://plos.figshare.com/articles/figure/NK_cell_degranulation_and_cytokine_release_in_response_to_SIV_infection_/5211238 %R 10.1371/journal.ppat.1006506.g005 %2 https://ndownloader.figshare.com/files/8972512 %K CD 107a %K TNF α upregulation %K HLA-DR %K NK cell activation %K NK cell responses %K NK cells %K Mamu-Bw 4 molecules %K interactions influence NK cell responses %K SIV infection %K KIR 3DL %K CD 16 %K KIR 3DL upregulation %K MHC class I-defined rhesus macaques Natural killer cells %K CD 56 %K polymorphic killer-cell immunoglobulin-like receptors %K gut-associated lymphoid tissues %K MHC class %X

Longitudinal changes in the upregulation of CD107a (A & C) and TNFα (B & D) are shown for KIR3DL01+, KIR3DL05+ and KIR3DL01-05- NK cells in response to stimulation with 721.221 cells (A & B) or without 721.221 cell stimulation (C & D). PBMCs were incubated overnight with or without 721.221 cells in the presence of an antibody to CD107a, and stained the following day with antibodies to differentiate KIR3DL01+, KIR3DL05+ and KIR3DL01-05- NK cells and for intracellular accumulation of TNFα. Representative gating for CD107a and TNFα staining is shown in S6 Fig. The mean and standard error (error bars) are plotted for the each NK cell population. Significance values for acute (weeks 1–4) and chronic (weeks 6–24) infection compared to pre-infection (week 0) are indicated with asterisks color-coded to the corresponding cell population (p<0.05*, p<0.01**, p< 0.005*** & p<0.001****, mixed effects models).

%I PLOS Pathogens