TY - DATA T1 - Longitudinal changes in NK and T cell counts in peripheral blood in response to SIV infection. PY - 2017/07/14 AU - Moritz Ries AU - Matthew R. Reynolds AU - Ksenia Bashkueva AU - Kristin Crosno AU - Saverio Capuano III AU - Trent M. Prall AU - Roger Wiseman AU - David H. O’Connor AU - Eva G. Rakasz AU - Hajime Uno AU - Jeffrey D. Lifson AU - David T. Evans UR - https://plos.figshare.com/articles/figure/Longitudinal_changes_in_NK_and_T_cell_counts_in_peripheral_blood_in_response_to_SIV_infection_/5211217 DO - 10.1371/journal.ppat.1006506.g001 L4 - https://ndownloader.figshare.com/files/8972500 KW - CD 107a KW - TNF α upregulation KW - HLA-DR KW - NK cell activation KW - NK cell responses KW - NK cells KW - Mamu-Bw 4 molecules KW - interactions influence NK cell responses KW - SIV infection KW - KIR 3DL KW - CD 16 KW - KIR 3DL upregulation KW - MHC class I-defined rhesus macaques Natural killer cells KW - CD 56 KW - polymorphic killer-cell immunoglobulin-like receptors KW - gut-associated lymphoid tissues KW - MHC class N2 - Absolute counts for total NK cells (A), CD8+ T cells (B), CD4+ T cells (C), KIR3DL01+ NK cells (D), KIR3DL05+ NK cells (E), KIR3DL01-05- NK cells (F), KIR3DL01+05+ NK cells (G), CD16+ NK cells (H), CD56+ NK cells (I), and CD16-CD56- NK cells (J) were monitored by staining whole blood and PBMCs as described in the methods. Individual (K) and geometric mean (L) SIV RNA loads in plasma are shown for Mamu-A1*002+ (blue) and–A1*002- (red) animals. Gating strategies for determining absolute lymphocyte counts in blood and the percentages of PBMCs expressing CD16, CD56, KIR3DL01 and KIR3DL05 are shown in S1 and S4 Figs. Viral loads were measured using a qRT-PCR assay with a detection threshold of 30 copies/ml (dotted line) and error bars indicate 95% CI for geometric mean values. Statistics were calculated using a mixed effects model by comparing results from acute (week 1–4) and chronic (weeks 6–24) infection to pre-infection (week 0) (p<0.05 *, p<0.01**, p< 0.005*** & p<0.001****). ER -