TY - DATA T1 - Susceptibility of human DGAT2 activity to cysteine-specific modifying reagents. PY - 2017/07/11 AU - Sunhee Jung AU - Miri Choi AU - Kwangman Choi AU - Eun Bin Kwon AU - Mingu Kang AU - Dong-eun Kim AU - Hyejeong Jeong AU - Janghwan Kim AU - Jong Heon Kim AU - Mun Ock Kim AU - Sang-Bae Han AU - Sungchan Cho UR - https://plos.figshare.com/articles/figure/Susceptibility_of_human_DGAT2_activity_to_cysteine-specific_modifying_reagents_/5196610 DO - 10.1371/journal.pone.0181076.g001 L4 - https://ndownloader.figshare.com/files/8870746 KW - DGAT 2 KW - NEM KW - IA KW - DGAT 2 activity KW - DTT KW - disulfide crosslinking KW - ROS-related chemicals KW - role KW - GPAT KW - TG biosynthesis KW - Human DGAT 2 activity KW - cysteine residues Diacylglycerol acyltransferases KW - H 2 O 2 N2 - (A) Membrane extracts from human DGAT2-overexpressing Sf9 insect cells were treated with indicated concentrations of NEM or IA. Human DGAT2 activity was measured by using the conventional extraction-based in vitro DGAT assay. The relative DGAT2 activity in percentage was calculated by setting the value from DMSO-treated sample to 100%. (B) Selective inhibitory effect of NEM on human DGAT2 activity compared to that on human DGAT1 and GPAT1. Membrane extracts from human DGAT2-, DGAT1-, or GPAT1-overexpressing Sf9 insect cells were treated with indicated concentrations of NEM or DMSO. Human DGAT1, DGAT2, and GPAT1 activity was measured by using the conventional extraction-based in vitro assays which are described in detail in the Materials and Methods section. The relative enzyme activity in percentage was calculated by setting the value from DMSO-treated sample to 100%. The mean values and standard deviations were determined from four independent assays. ER -