%0 Figure %A Tsai, Yi Ting %A Salzman, Valentina %A Cabruja, Matías %A Gago, Gabriela %A Gramajo, Hugo %D 2017 %T Fig S3 from Role of long-chain acyl-CoAs in the regulation of mycolic acid biosynthesis in mycobacteria %U https://rs.figshare.com/articles/figure/Fig_S3_from_Role_of_long-chain_acyl-CoAs_in_the_regulation_of_mycolic_acid_biosynthesis_in_mycobacteria/5183314 %R 10.6084/m9.figshare.5183314.v1 %2 https://ndownloader.figshare.com/files/8835691 %K mycobacterium %K mycolic acid %K acyl-CoA %K isoniazid %K transcriptional regulation %X Effect of C26-CoA on the formation of C20-CoA/ His6-MabRMT /PfasII complex. His6-MabRMT was preincubated during 5 min with C26-CoA in the presence of poly-dIdC, prior to the addition of C20-CoA and 5 min later, PfasII probe was added (last 2 lanes). The His6-MabRMT-DNA complex formation was also assayed with the individual addition of different concentrations of C20-CoA (lanes 3-6) or C26-CoA (lanes 7-10), as controls of the experiment. %I The Royal Society