Testing possible explanations for high phage titer in cultures of Lula lysogens.
RotmanElla
AmadoLuciana
KuzminovAndrei
2013
<p><b>A.</b> UV-sensitivity of lysogens. Strains: wild type non-lysogen, AB1157; Lambda lysogen, EL104; Lula lysogen, EL103. The data points are means of 3–4 independent measurements, done on different days, ± SEM. <b>B.</b> Phage titer in saturated cultures of wild type (EL103 and EL104) and Δ<i>recA</i> lysogens (Lula, EL105; Lambda, EL106). The data points are means of five independent measurements, done on the same day, ± SEM. <b>C.</b> Cell titer versus Lula titer in the culture of a Lula lysogen (EL103) as a test for possible quorum sensing. Note that X axis is in log scale, as the left Y axis, but the right Y axis (for the lower curve) is linear. <b>D.</b> Stability of Lambda in saturated cultures of Lambda lysogen (EL104) and non-lysogen (AB1157). The data points are means of 3 independent measurements, done on different days, ± SEM. <b>E.</b> Stability of Lula in saturated cultures of Lula lysogen (EL103) and non-lysogen (AB1157). The data points are means of 3 independent measurements, done on different days, ± SEM. <b>F.</b> Stability of Lambda and Lula against aerosolation. The loss of phage titer in either stationary or rapidly shaking (to generate frothing) 1% NaCl suspensions was determined at two time points.</p>