TY - DATA T1 - Confocal microscopy images of immunofluorescence staining for Bradyrhizobium sp. prepared from pure culture and plant nodules. PY - 2017/06/27 AU - Nguyen Xuan Vu AU - Natcha Pruksametanan AU - Witsanu Srila AU - Watcharin Yuttavanichakul AU - Kamonluck Teamtisong AU - Neung Teaumroong AU - Nantakorn Boonkerd AU - Panlada Tittabutr AU - Montarop Yamabhai UR - https://plos.figshare.com/articles/figure/Confocal_microscopy_images_of_immunofluorescence_staining_for_i_Bradyrhizobium_i_sp_prepared_from_pure_culture_and_plant_nodules_/5149210 DO - 10.1371/journal.pone.0179983.g007 L4 - https://ndownloader.figshare.com/files/8771782 KW - phage enzyme-linked immunosorbent assay KW - phage ELISA KW - rabbit single-chain fragment KW - detection KW - plant nodules KW - phage-displayed scFv antibody KW - nitrogen-fixing bacteria KW - Bradyrhizobium sp KW - host range soil bacteria KW - bacteroid forms KW - free-living KW - DOA 9 KW - polyclonal antibody KW - phage display technology N2 - Immunofluorescence staining of various bacterial targets in both pure culture and nodule forms are shown. The bacteria tested include Bradyrhizobium sp. strains DOA9 (A), SUTN9-2 (B), SUTN1-12 (C), and USDA110 (D). The bacterial samples were stained with various antibodies, i.e., rabbit polyclonal antibody, two clones of phage-displayed rabbit scFv, i.e., RB9 and RG8, which contained identical DNA sequence. Phage-displayed scFv antibody clone 3C1 that bind aflatoxin B1 was used as a negative control. Green spots indicated the green fluorescent staining of phage display scFv or polyclonal antibody, using secondary antibody conjugated to FITC. Plant cell walls were stained with a blue fluorophore (Calcofluor white M2R) and emitted blue color. The bacteroid are shown as green spots inside a blue plant cavity. Scale bar is 10 μm at 80× magnification, and 100 μm at 10× magnification for pure culture and nodule samples, respectively. ER -