%0 Figure %A Vu, Nguyen Xuan %A Pruksametanan, Natcha %A Srila, Witsanu %A Yuttavanichakul, Watcharin %A Teamtisong, Kamonluck %A Teaumroong, Neung %A Boonkerd, Nantakorn %A Tittabutr, Panlada %A Yamabhai, Montarop %D 2017 %T Confocal microscopy images of immunofluorescence staining for Bradyrhizobium sp. prepared from pure culture and plant nodules. %U https://plos.figshare.com/articles/figure/Confocal_microscopy_images_of_immunofluorescence_staining_for_i_Bradyrhizobium_i_sp_prepared_from_pure_culture_and_plant_nodules_/5149210 %R 10.1371/journal.pone.0179983.g007 %2 https://ndownloader.figshare.com/files/8771782 %K phage enzyme-linked immunosorbent assay %K phage ELISA %K rabbit single-chain fragment %K detection %K plant nodules %K phage-displayed scFv antibody %K nitrogen-fixing bacteria %K Bradyrhizobium sp %K host range soil bacteria %K bacteroid forms %K free-living %K DOA 9 %K polyclonal antibody %K phage display technology %X

Immunofluorescence staining of various bacterial targets in both pure culture and nodule forms are shown. The bacteria tested include Bradyrhizobium sp. strains DOA9 (A), SUTN9-2 (B), SUTN1-12 (C), and USDA110 (D). The bacterial samples were stained with various antibodies, i.e., rabbit polyclonal antibody, two clones of phage-displayed rabbit scFv, i.e., RB9 and RG8, which contained identical DNA sequence. Phage-displayed scFv antibody clone 3C1 that bind aflatoxin B1 was used as a negative control. Green spots indicated the green fluorescent staining of phage display scFv or polyclonal antibody, using secondary antibody conjugated to FITC. Plant cell walls were stained with a blue fluorophore (Calcofluor white M2R) and emitted blue color. The bacteroid are shown as green spots inside a blue plant cavity. Scale bar is 10 μm at 80× magnification, and 100 μm at 10× magnification for pure culture and nodule samples, respectively.

%I PLOS ONE