10.1371/journal.pone.0179983.g007
Nguyen Xuan Vu
Nguyen Xuan
Vu
Natcha Pruksametanan
Natcha
Pruksametanan
Witsanu Srila
Witsanu
Srila
Watcharin Yuttavanichakul
Watcharin
Yuttavanichakul
Kamonluck Teamtisong
Kamonluck
Teamtisong
Neung Teaumroong
Neung
Teaumroong
Nantakorn Boonkerd
Nantakorn
Boonkerd
Panlada Tittabutr
Panlada
Tittabutr
Montarop Yamabhai
Montarop
Yamabhai
Confocal microscopy images of immunofluorescence staining for <i>Bradyrhizobium</i> sp. prepared from pure culture and plant nodules.
Public Library of Science
2017
phage enzyme-linked immunosorbent assay
phage ELISA
rabbit single-chain fragment
detection
plant nodules
phage-displayed scFv antibody
nitrogen-fixing bacteria
Bradyrhizobium sp
host range soil bacteria
bacteroid forms
free-living
DOA 9
polyclonal antibody
phage display technology
2017-06-27 17:41:05
Figure
https://plos.figshare.com/articles/figure/Confocal_microscopy_images_of_immunofluorescence_staining_for_i_Bradyrhizobium_i_sp_prepared_from_pure_culture_and_plant_nodules_/5149210
<p>Immunofluorescence staining of various bacterial targets in both pure culture and nodule forms are shown. The bacteria tested include <i>Bradyrhizobium</i> sp. strains DOA9 (<b>A</b>), SUTN9-2 (<b>B</b>), SUTN1-12 (<b>C</b>), and USDA110 (<b>D</b>). The bacterial samples were stained with various antibodies, i.e., rabbit polyclonal antibody, two clones of phage-displayed rabbit scFv, i.e., RB9 and RG8, which contained identical DNA sequence. Phage-displayed scFv antibody clone 3C1 that bind aflatoxin B1 was used as a negative control. Green spots indicated the green fluorescent staining of phage display scFv or polyclonal antibody, using secondary antibody conjugated to FITC. Plant cell walls were stained with a blue fluorophore (Calcofluor white M2R) and emitted blue color. The bacteroid are shown as green spots inside a blue plant cavity. Scale bar is 10 μm at 80× magnification, and 100 μm at 10× magnification for pure culture and nodule samples, respectively.</p>