Enhanced Phototherapy
by Nanoparticle-Enzyme via Generation
and Photolysis of Hydrogen Peroxide
Kaiwen Chang
Zhihe Liu
Xiaofeng Fang
Haobin Chen
Xiaoju Men
Ye Yuan
Kai Sun
Xuanjun Zhang
Zhen Yuan
Changfeng Wu
10.1021/acs.nanolett.7b01382.s001
https://acs.figshare.com/articles/journal_contribution/Enhanced_Phototherapy_by_Nanoparticle-Enzyme_via_Generation_and_Photolysis_of_Hydrogen_Peroxide/5144149
Light has been widely
used for cancer therapeutics such as photodynamic therapy (PDT) and
photothermal therapy. This paper describes a strategy called enzyme-enhanced
phototherapy (EEPT) for cancer treatment. We constructed a nanoparticle
platform by covalent conjugation of glucose oxidase (GOx) to small
polymer dots, which could be persistently immobilized into a tumor.
While the malignant tumors have high glucose uptake, the GOx efficiently
catalyzes the glucose oxidation with simultaneous generation of H<sub>2</sub>O<sub>2</sub>. Under light irradiation, the in situ generated
H<sub>2</sub>O<sub>2</sub> was photolyzed to produce hydroxyl radical,
the most reactive oxygen species, for killing cancer cells. In vitro
assays indicated that the cancer cells were destroyed by using a nanoparticle
concentration at 0.2 μg/mL and a light dose of ∼120 J/cm<sup>2</sup>, indicating the significantly enhanced efficiency of the
EEPT method when compared to typical PDT that requires a photosensitizer
of >10 μg/mL for effective cell killing under the same light
dose. Furthermore, remarkable inhibition of tumor growth was observed
in xenograft-bearing mice, indicating the promise of the EEPT approach
for cancer therapeutics.
2017-06-14 00:00:00
nanoparticle
Hydrogen Peroxide Light
therapy
cancer cells
light dose
reactive oxygen species
tumor
glucose
cancer therapeutics
H 2 O 2
EEPT
PDT
GOx