10.6084/m9.figshare.5129755.v1 de Toro-Martín J. de Toro-Martín J. Guénard F. Guénard F. Tchernof A. Tchernof A. Deshaies Y. Deshaies Y. Pérusse L. Pérusse L. Biron S. Biron S. Lescelleur O. Lescelleur O. Biertho L. Biertho L. Marceau S. Marceau S. Vohl M.-C. Vohl M.-C. Supplementary Material for: A CpG-SNP Located within the <b><i>ARPC3</i></b> Gene Promoter Is Associated with Hypertriglyceridemia in Severely Obese Patients Karger Publishers 2016 Metabolic syndrome Triglycerides Obesity Visceral adipose tissue Actin-related protein 2/3 complex subunit 3 CpG-SNP 2016-04-08 00:00:00 Dataset https://karger.figshare.com/articles/dataset/Supplementary_Material_for_A_CpG-SNP_Located_within_the_b_i_ARPC3_i_b_Gene_Promoter_Is_Associated_with_Hypertriglyceridemia_in_Severely_Obese_Patients/5129755 <b><i>Aims:</i></b> To test the potential association of cytosine-phosphate-guanine dinucleotides (CpG)-single-nucleotide polymorphisms (SNPs) located within actin-related protein 2/3 complex subunit 3 (<i>ARPC3</i>), a gene recently linked to adipogenesis and lipid accumulation, with metabolic syndrome (MetS) features in severely obese patients. <b><i>Methods:</i></b> Prioritized SNPs within the <i>ARPC3</i> locus were genotyped and tested for associations with MetS features in a cohort of 1,749 obese patients with and without MetS. Association testing with CpG methylation levels was performed in a methylation sub-cohort of 16 obese men. <b><i>Results:</i></b> A significant association was found between the CpG-SNP rs3759384 (C>T) and plasma triglyceride (TG) levels (false discovery rate-corrected p = 3.5 × 10<sup>-2</sup>), with 0.6% of the phenotypic variance explained by the CpG-SNP, and with TT homozygotes showing the highest plasma TG levels (1.89 mmol/l). The carriers of the rs3759384 T allele also showed a significant decrease in methylation levels of the <i>ARPC3</i> promoter-associated CpG site cg10738648 in both visceral adipose tissue and blood. <i>ARPC3</i> expression levels showed a strong correlation with plasma TG levels (r = 0.70; p = 0.02). <b><i>Conclusions:</i></b> The increased plasma TG levels found in homozygous rs3759384 T allele carriers argue for a relevant role of this CpG-SNP in lipid management among obese individuals, which may be driven by an epigenetic-mediated mechanism.